Primary Identification of Triterpene Compounds in Chizhi (Ganoderma lucidum) by HPLC-APCI-MS

FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (7): 367-372.

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Primary Identification of Triterpene Compounds in Chizhi (Ganoderma lucidum) by HPLC-APCI-MS

CHEN Jun-Hui, LIU Jie, WU Feng-Qi, LI Xian-Chun, WANG Xiao-Ru

1.College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266003, China ; 2.Qingdao Key Laboratory on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography, State Oceanic Administration of People′s Repubic of China, Qingdao 266061, China;3.Key Laboratory of Analytical Science, Ministry of Education, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Online:2007-07-15 Published:2011-10-24

Abstract

Abstract: High performance liquid chromatography (HPLC)-atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) was applied to analyze the triterpenoids in extracts of Ganoderma lucidum. The extraction conditions are: Hypersil C4 (4.6 mm × 150 mm) column with gradient elution with acetonitrile and water (2.0% acetic acid) as mobile phase, flow-rate 0.8 ml/min, and room temperature about 25 ℃. The detection wavelength is 253 nm and the UV spectra of peaks are obtainedwith a photodiode array detector (DAD). On-line APCI-MS in positive and negative mode was used to get the mass spectrum of the analyte. Thirty-two components of Ganoderma lucidum are primarily identified by comparison of the UV spectra and mass spectrum according to published literatures.

Key words: Ganoderma lucidum, triterpenoids, HPLC, APCI-MS

CHEN Jun-Hui, LIU Jie, WU Feng-Qi, LI Xian-Chun, WANG Xiao-Ru. Primary Identification of Triterpene Compounds in Chizhi (Ganoderma lucidum) by HPLC-APCI-MS[J]. FOOD SCIENCE, 2007, 28(7): 367-372 https://doi.org/

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Primary Identification of Triterpene Compounds in Chizhi (Ganoderma lucidum) by HPLC-APCI-MS

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