Abstract: Detection of efficient component in bitter melon beer was established by HPLC. The bitter melon saponin was extracted by transonogram-assisted using 70% ethyl alcohol, then was purified by macroporous resin, and the straight of bitter melon saponin was obtained. Bitter melon saponin in bitter melon beer was compared with standard substance made by our laboratory. An RP-HPLC method was set up, using ODS-3 column, acetonitrile: water (55:45, V/V) as mobile phase with a flow rate of 1.0 ml/min at the detection wavelength of 205 nm, the qualitative and the quantitative analyses were carried out based on the retention time and peak area respectively. The results showed that the total content of bitter melon saponin in bitter melon beer is 22.16 μ g/ml, RSD (relative standard deviation) is less than 4%, recovery is between 97.53%～100.32%. This method is excellent reproducibility and the is operation simple. It is a perfect detection method.

Key words: HPLC, detection, bitter melon beer, bitter melon saponin