FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (7): 446-449.

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Analysis of Monosacchaaride Composition in Tremella Polysaccharides by Non-derivatization High Performance Liquid Chromatography

 YAN  Jun, GUO  Xiao-Qiang, WU  Xiao-Yong, XU  Guang-Yu, HU  Xiao-Xiao, TAN  Zhou-Jun, GOU  Xiao-Jun   

  1. 1.Key Laboratory of Medical Microbial Resources, Chengdu University, Chengdu 610106, China; 2.Laboratory of Traditional Chinese Medicine, Chengdu University, Chengdu 610106, China
  • Online:2007-07-15 Published:2011-10-24

Abstract: This article is discussing a simple and sensitive high performance liquid chromatographic method(HPLC) for the analysis of monosacchoride composition in polysaccharides. Five monosaccharides are separated by non-derivatization HPLC with RI monitor. By means of HPLC analysis rhamnose, glucose, fructose, xylose, galactose five kinds of monosaccharides, the best separation condition of the monosaccharide is obtained. The monosaccharide composition of Tremella polysaccharide is determined by a data analysis method which is developed to ensure accuracy and reproducibility.

Key words: HPLC, Tremella polysaccharide, monosaccharide