Abstract:  With corn embryo of coproduct from corn starch produced with wet method as material, superoxide dismutase(SOD) was extracted through sonication after supercritical CO2 fluid extraction for defating and low temperature crushing. The results showed that optimum conditions are solid to liquid 1:25, the time 6 s, the times 40 and the interval time 2 s. The crude extract was purified through thermal denaturation and acetone sediment and Sephadex G-75 column chromatography. The enzyme obtained activity recovery was 40.56%, the removal rate of protein was 99.36%, the specific activity was 3079.41 U/mg, and pure the purify multiple was 63.09. The scanning results of ultraviolet spectrophotometer showed that the peak of ultraviolet absorption of SOD from corn embryo was at 260 nm, the same to standard preparation. It was that the superoxide dismutase pure of corn embryo is Cu/Zn-SOD.

Key words: corn, embryo, superoxide dismutase, ultrasonics-assistant extraction