Study on Composition and Structure of Proanthocyanidins from Sea Buckthorn (Hippophae rhamnoides) Seeds

Abstract

Abstract: The monomeric and oligomeric proanthocyanidin fractions were analyzed by HPLC-ESI-MASS assay. The polymeric proanthocyanidins were fractionated by Sephadex LH-20 column chromatograph and their chemical constitution were also studied by acid-catalysed degradation in the presence of benzyl mercaptan. Four monomers were identified as catechin, epicatechin, gallocatechin and epigallocatechin. Eight dimers including three dimeric procyanidins, one dimeric prodelphinidin and four mixed dimers were detected and their structures were partly elucidated by the detection of the pseudomolecular ions and main ion fragmentations. The results also showed that the polymers are highly heterogenous, with catechin, epicatechin, gallocatechin and epigallocatechin all being constituent components of both the extension as well as the terminal units. The extension units are mainly gallocatechin, whereas terminal units are mainly gallocatechin and epigallocatechin for all fractions. The polymeric fractions with mDP 9.1, 13.2 and 17.0 represent the three major polymeric fractions accountingfor 74.7% of total polymers.

Key words: sea buckthorn (Hippophae rhamnoides) seeds, proanthocyanidins, HPLC-ESI-MASS

FAN Jin-Ling, LUO Lei, YAO Ping, ZHU Wen-Xue. Study on Composition and Structure of Proanthocyanidins from Sea Buckthorn (Hippophae rhamnoides) Seeds[J]. FOOD SCIENCE, 2008, 29(1): 244-249.

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Study on Composition and Structure of Proanthocyanidins from Sea Buckthorn (Hippophae rhamnoides) Seeds

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