Abstract: With orthogonal test design, to optimize bolting of medium and culture conditions with co-culture of Panax ginseng and Daucus carota, the saponin contents of Panax ginseng and co-culture were simultaneously compared. The results showed that the callus of the co-culture of Panax ginseng and Daucus carota grow well on the condition of 23 ℃ and 1000 lx with the optimal medium MS+2,4-D 3 mg/L+IBA 2 mg/L+KT 0.5 mg/L. After 25 d culture, the saponin content of co-culture is 6.25% higher than that of Panax ginseng callus. It settles foundation for protoplast fusion of Panax ginseng and Daucus carota as to obtain trophic vegetables.

Key words: Panax ginseng, Daucus carota, co-culture, Panax saponin