Abstract: Aspergillus flavus standard strain DNAS were isolated by using three extraction methods and three pretreatment methods of mycelia for benzyl chloride extraction method. The gel electrophoresis results showed that the DNA isolated by using benzyl chloeide method and treated with RNase is the best after the mycelia are freezed overnight at -80 ℃ or grinded with liquid nitrogen. Two pairs of primes were synthesized to amplify aflR gene. The PCR products are not false positive which is confirmed by nest-PCR. The expected restriction endonuclease analysis results of the PCR products with HincⅡand PvuⅡwere achieved,which showed that the PCR-RFLP detection method is feasible for Aspergillus flavus.

Key words: Aspergillus flavus, DNA, PCR-RFLP