Abstract: Tricholoma mongolicum Imai after supercritical CO2 fluid extracted was used as material to extract polysaccharide through microwave. The optimum conditions of extract polysaccharide from Tricholom amongolicum Imai were determined through single factor and orthogonal test: microwave power 300 W, microwave processing time 4 min, solid-liquid ratio 1:30, material grade size 0.147 mm. Tricholom amongolicum Imai polysaccharides were extracted by above conditions, the extraction rate of polysaccharide was 31.05%.

Key words: Tricholoma mongolicum Imai, polysaccharide, microwave, supercritical CO2 fluid extraction