FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (4): 352-354.

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Determination of Cordycepin from Cultured Cordyceps sinensis by HPLC-DAD

 SONG  Jiang-Feng, LIU  Chun-Quan, LI  Da-Jing, JIN  Bang-Quan   

  1. 1.Jinling College, Nanjing Normal, University; 2. Institute of Atomic Energy in Application for Agriculture, Jiangsu Academy of Agricultural Sciences
  • Online:2008-04-15 Published:2011-08-24

Abstract: Determination of cordycepin in cultured Cordyceps sinensis by HPLC-DAD was showed. Eclipse XDB C18 column (4.6 mm×150 mm, 5μm) was used with water: ethanol: formic acid (95:4:1, V/V/V) as mobile phase and ultraviolet detection wavelength was 260 nm. The results showed that the average recovery of cordycepin is 100.8%, with RSD 1.88%. This method is simple and reliable.

Key words: Cordyceps sinensis, cordycepin, HPLC-DAD