Abstract: DNA was extracted from spores of Tremella fuciformis by phenol-chloroform method, CTAB (cetyltrimethyl ammonium bromide) method, proteinase K method, benzyl chloride method and salting out method respectively. The purity and yield of the DNA were determined by A260/A280 respectively. The quality of the DNA were evaluated with RAPD (random amplified polymerphic DNA technology) and restrict endonuclease digestion. Results showed that there are some differences in purity and yield among those 5 methods. Both benzyl chloride method and proteinase K method have better yield, but the purity of the DNA by CTAB method is better than that of others. In a word, the CTAB method is most suitable for extracting genomic DNA from spores of Tremella fuciformis.

Key words: Tremella fuciformis, spores, DNA extraction, RAPD, restriction endonuclease digestion