Comparison of Five Methods for Genomic DNA Extraction from Spores of Tremella fuciformis

Abstract

Abstract: DNA was extracted from spores of Tremella fuciformis by phenol-chloroform method, CTAB (cetyltrimethyl ammonium bromide) method, proteinase K method, benzyl chloride method and salting out method respectively. The purity and yield of the DNA were determined by A260/A280 respectively. The quality of the DNA were evaluated with RAPD (random amplified polymerphic DNA technology) and restrict endonuclease digestion. Results showed that there are some differences in purity and yield among those 5 methods. Both benzyl chloride method and proteinase K method have better yield, but the purity of the DNA by CTAB method is better than that of others. In a word, the CTAB method is most suitable for extracting genomic DNA from spores of Tremella fuciformis.

Key words: Tremella fuciformis, spores, DNA extraction, RAPD, restriction endonuclease digestion

LIU Juan, MA Ai-Min, YANG Jiang-Tao, LI Da-Fang. Comparison of Five Methods for Genomic DNA Extraction from Spores of Tremella fuciformis[J]. FOOD SCIENCE, 2008, 29(6): 248-251.

[1]	REN Hongrong, LI Miaoyun, ZHU Yaodi, ZHAO Gaiming, ZHAO Lijun, WU Huilin, XIAO Kang, CUI Wenming. Recent Progress in Hazards and Control of Clostridium perfringens in Foods [J]. FOOD SCIENCE, 2021, 42(7): 352-359.
[2]	ZHOU Mengyue, XING Ranran, WANG Nan, GE Yiqiang, CHEN Ying. Comparison of Six Methods for Extraction of Genomic DNA from Edible Spices [J]. FOOD SCIENCE, 2021, 42(16): 246-253.
[3]	DU Yan, CHEN Fusheng, CHEN Chen, LIU Ying. Comparative Evaluation of DNA Extraction Methods for Detection of Transgenic Soybean Protein and Analysis of lectin Gene Degradation during Soybean Protein Extraction [J]. FOOD SCIENCE, 2020, 41(4): 102-111.
[4]	LIU Tingting, YANG Jiadan, CAO Chenyu, ZHANG Yanrong. Rheological and Gelling Properties of Tremella fuciformis Polysaccharide and Gellan Gum Mixtures [J]. FOOD SCIENCE, 2019, 40(17): 72-78.
[5]	BAI Yan, GE Yujun, XIANG Yingchun, LI Yuan, DING Tian, HU Yaqin. Progress in Research on the Efficacies and Mechanisms of Action of Various Non-thermal Sterilization Technologies in Inactivation of Microbial Spores in Foods [J]. FOOD SCIENCE, 2019, 40(15): 314-322.
[6]	YANG Jiadan, LIU Tingting, ZHANG Shanshan, CAO Chenyu, WANG Dawei. Optimization of Microwave-Assisted Extraction and Rheological and Gelling Properties of Polysaccharide from Tremella fuciformis [J]. FOOD SCIENCE, 2019, 40(14): 289-295.
[7]	ZHU Kai, KANG Huaibin, WANG Deguo. Visual Detection of Pork Components in Common Meat Products by Loop-Mediated Isothermal Amplification (LAMP) Method [J]. FOOD SCIENCE, 2019, 40(12): 296-302.
[8]	ZHANG Qiuxue, LIU Xiaochan, ZHU Zongtao, WAN Feng, SUN Sirui, JIA Fangfang, MENG Xiangchen. Isolation and Diversity Analysis of Bifidobacterium longum from Infant Faeces [J]. FOOD SCIENCE, 2017, 38(24): 8-14.
[9]	CHEN Gang, ZHAN Yong, YANG Yong, WU Zhen, XIE Huichuan, SHI Wenjuan, CHAI Jiayan, GONG Hurong, LUO Yang. Effect of Cultivation Temperature on Extracellular Enzyme Activities and Nutritional Quality Characteristics of Tremella fuciformis [J]. FOOD SCIENCE, 2017, 38(23): 113-120.
[10]	LIU Yang, ZHU Hanyu, XU Danyun, ZHANG Lijiao, MA Aimin. Development of an Efficient Method for Total Protein Extraction from Yeast-Like Conidia of Tremella fuciformis [J]. FOOD SCIENCE, 2016, 37(9): 35-39.
[11]	LIU Chunyan, ZHANG Guocai, CHENG Fangzhi, ZHANG Guozhen, ZHAO Bo, LIN Liannan. Optimization of Disruption of Ganoderma lucidum Spores and Antitumor Effect of the Broken Spores [J]. FOOD SCIENCE, 2016, 37(14): 51-55.
[12]	CAI Linxi, LIU Ke, YUAN Yiping, Lü Jianlei, WANG Yalei, BAO Haiying. Formulation Optimization of Odor-Removing Candy Containing Medicinal Fungi [J]. FOOD SCIENCE, 2016, 37(10): 47-53.
[13]	QU Hongyan, GUO Chengjin*. Ultraviolet-Induced Mutagenesis of Cordyceps sinensis and Cordyceps militaris Protoplast Fusant [J]. FOOD SCIENCE, 2015, 36(5): 120-125.
[14]	WANG Fanhua1, MA Wutiao2, TAN Qingyun1, LIU Weixian2, SUN Tian2, DU Bing3, YANG Gongming3, YI Jicai1,*. DNA Extraction for RAPD Analysis of Dried Tremella fuciformis [J]. FOOD SCIENCE, 2015, 36(4): 172-175.
[15]	GAO Sujuan, LI Zhiyong, GAO Dongwei, LIU Jin. A Review on Grape (Vitis vinifera L.) DNA Extraction and Molecular Identification for Wines [J]. FOOD SCIENCE, 2015, 36(15): 282-287.

Comparison of Five Methods for Genomic DNA Extraction from Spores of Tremella fuciformis

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments