Study on Detection Conditions Activity of IgG from Bovine Serum by Rocket Electrophoresis

Abstract

Abstract: The study aimed to establish a rapid method,rocket electrophoresis(RE) to detect content and activity of IgG from bovine serum. The results showed that the optimal conditions of RE are as follows:3.0%(V/V) antibody,adopting barbital sodium-HCl buffer(pH 8.6),hydroformylation with glutaraldehyde-barbital sodium(5%) at the proportion 1:4(V:V),and elution of non-specific protein with 0.01 mol/L PBST(at pH 7.4). RE is an effective way with good linearity results,correlation coefficient 0.9947,and also a simple and low-cost method,which can be used for detecting IgG during practical industrial product process.

Key words: immunoglobulin, rocket electrophoresis, immunocompetence

LIU Ai-Guo, WU Zi-Jian, XU Qing, YANG Gui-You. Study on Detection Conditions Activity of IgG from Bovine Serum by Rocket Electrophoresis[J]. FOOD SCIENCE, 2008, 29(8): 537-540.

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Study on Detection Conditions Activity of IgG from Bovine Serum by Rocket Electrophoresis

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