Abstract:

To generate a monoclonal antiboby specific to both vardenafil and its analogues, glutaraldehyde was used as conjugating reagent to link vardenafil to immunogen, leaving the common structure of vardenafil and its analogues exposed on the surface of conjugates as major epitode. Through optimization, immunogen with a molar ratio of 6.8:1 could induce a high titer specific antibody in sera of mice. By using hybridomas technique, 4 monoclonal antibodies with the ability to recognize vardenafil and its analogue piperidenafil were obtained.

Key words: phosphodiesterase-5 inhibitors, vardenafil, illegal additives, artifacial antigen, immunoassay