Abstract:

A synchronous spectrofluorimetric method is described for the determination of tryptophane, tyrosine and phenylalanine in food and drug injections in this paper. When Δλ was 70 nm for synchronous scanning, the characteristic peaks of tryptophane, tyrosine and phenylalanine appeared at 280, 228 nm and 206 nm, respectively. Tyrosine and phenylalanine could be directly quantified from their synchronous signals. The synchronous signal of tryptophane was slightly interfered by that of tyrosine, the interference from tyrosine could be effectively removed by the first order derivation synchronous fluorimetry. The detection limit was 6.9 ×10-9 mol/L for tryptophane, 3.8 ×10-8 mol/L for tyrosine and 4.2 × 10-7 mol/L for phenylalanine. No preliminary separation was needed for the determination by this method. It has been successfully applied to the analysis of beer samples and compound amino acids injection.

Key words: synchronous fluorescence spectrometry, derivation synchronous fluorescence spectrometry, aromatic amino acid, simultaneous determination