Abstract:

To eliminate the allergens in Litopenaeus vannamei, the meat of this shrimp was hydrolyzed separately with trypsin and papain, and their respective hydrolysis conditions were optimized for achieving minimum optical density at 492 nm (OD492 nm) in the indirect ELISA determination of allergen elimination. The optimal conditions for trypsin hydrolysis were determined as follows: pH 8.0; enzyme/substrate mass ratio 1:100; hydrolysis temperature 45 ℃; substrate concentration 5 g/100 mL; and hydrolysis duration 3 h, and the resultant OD492 nm value was 0.085. pH of 6.5, enzyme/substrate mass ratio of 1:100, hydrolysis temperature of 60 ℃, substrate concentration of 5 g/100 mL and hydrolysis duration of 3 h were found optimal for papain hydrolysis, and the minimum OD492 nm value under these conditions was as low as 0.049.

Key words: Litopenaeus vannamei, allergen elimination, ELISA