FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 244-247.doi: 10.7506/spkx1002-6630-201021055

• Bioengineering • Previous Articles     Next Articles

Full-length cDNA Cloning of Glycosyltransferase Family from Rhodiola sachalinensis

YU Han-song1，ZHANG Ji-xing2，LI Yan-fang3，MA Lan-qing4,*，HU Yao-hui1,*   

  1. 1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China；
    2. College of Life Sciences, Inner Mongolia University for the Nationalities, Tongliao 028043, China；
    3. College of Plant Science, Jilin University, Changchun 130062, China；
    4. Key Laboratory of Urban Agriculture(North), Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China
  • Received:2010-06-30 Revised:2010-10-20 Online:2010-11-15 Published:2010-12-29
  • Contact: HU Yao-hui E-mail:huyaohui@vip.163.com

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Abstract:

In order to obtain the critical enzyme for the synthesis of Rhodiola sachalinensis glycoside, two putative UDPglycosyltransferase (UGT) cDNAs (GenBank accession number, EF508689和EU567325) were isolated from R. sachalinensi. The primers were designed for 3＇-rapid amplification of cDNA ends (RACE) based on the consensus hybrid oligonucleotide primers (CODEHOP) strategy by the Block Maker program. A full-length cDNA sequence (Genbank accession numbers: EF508689 and EU567325) was obtained through the amplification using a SMARTerTM RACE cDNA Amplification Kit (TaKaRa, Dalian, China), whereas the sequence obtained using a 5'-RACE system (version 2.0, InvitrogenTM Life Technologies) contained no full-open reading frame.The cloning efficient of two 5'-RACE systems was investigated. Results exhibited that TaKaRa SMARTerTM RACE cDNA Amplification Kit was more efficient in complete cDNA sequence cloning, compared with the InvitrogenTM 5'-RACE system.

Key words: glycosyltransferase, gene cloning, rapid amplification of cDNA ends (RACE), Rhodiola sachalinensis

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