Isolation, Purification and Molecular Weight Determination of Polysaccharides from

Abstract

Abstract: Polysaccharides from Actinidia arguta Sieb. et Zucc. were extracted by hot-water extraction, ethanol precipitation, protein removal by the Sevag method and then purified by DEAE-cellulose-52 ion exchange column chromatography. Four polysaccharide fractions were obtained. The best purification of Fraction Ⅱ was achieved by chromatographic separation on a Sephadex G-200 column (D1.1 cm × 100 cm) at a sample concentration of 10 g/L. As a result, a homogenous pure polysaccharide was obtained. The results of UV spectroscopic analysis revealed that the purified polysaccharide contained neither proteins nor nucleic acids. Moreover, the molecular weight of the purified polysaccharide was measured to be 83733 D.

Key words: Actinidia arguta Sieb. et Zucc., polysaccharide, isolation and purification, column chromatography, ultraviolet spectroscopy, molecular weight determination

CLC Number:

TS255.36

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