FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (20): 262-264.

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Development and Application of Loop-Mediated Isothermal Amplification (LAMP) Method for Detection of  Vibrio parahaemolyticus

  

  • Received:2011-08-11 Revised:2012-09-20 Online:2012-10-25 Published:2012-11-09
  • Contact: Chao-bo XUE E-mail:xuechaobo2001@163.com
  • Supported by:

    ;Grant No.2010C32005

Abstract: In order to establish a loop-mediated isothermal amplification (LAMP) method for rapid diagnosis of Vibrio vulnificus, 4 primers that can recognize the vvhA gene of Vibrio vulnificus were designed and used for LAMP assay. Results showed that this method was highly specific for Vibrio vulnificus. Among 13 amplified strains, Vibrio vulnificus was LAMP positive, and other strains were LAMP negative. Moreover, the detection procedure could be completed within 60 minutes and had a detection limit of 51 CFU/mL. Of 120 seafood samples detected by this method, 37 were positive, which was consistent with the result obtained by the NMKL method (No. 156. 2nd edition. 1997). Compared with the NMKL method, this LAMP method was greatly time saving and lowered instrumental and operational requirements, suggesting good practical applicability.

Key words: Vibrio vulnificus, loop-mediated isothermal amplification (LAMP), vvhA gene

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