Identification of Puffer Species by PCR-RFLP Analysis and Chip Bio-analysis System

Abstract

Abstract: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and chip bio-analysis system were used to identify five puffer species. A 464-bp fragment of mitochondrial cytochrome b gene was amplified by PCR and the products were digested with the restriction endonuclease DdeⅠ, Hae Ⅲ and Nla Ⅲ, respectively. The resulting fragments were further resolved on DNA chip. Five puffer species were successfully identified by this method. In conclusion, PCR-RFLP and chip bio-analysis system could provide a fast, easy, automatic and reliable analysis approach.

Key words: puffer, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), hip bio-analysis system, species identification

CLC Number:

S917.4
Q78

References

[1] ESPINEIRA M, VIEITES J M, SANTACLARA F J. Development of a genetic method for the identification of salmon, trout, and bream in seafood products by means of PCR-RFLP and FINS methodologies [J]. Eur Food Res Technol, 2009, 229: 785-793.
[2] SOTELO C G, CALO-MATA P, CHAPELA M J, et al. Identification of flatfish (Pleuronectiforme) species using DNA-based techniques [J]. J Agric Food Chem, 2001, 49(10): 4562-4569.
[3] LIN W F, HWANG D F. Application of PCR-RFLP analysis on species identification of canned tuna [J]. Food Control 2007, 18(9): 1050-1057.
[4] ESPINEIRA M, GONZALEZ-LAVIN N, VIEITES J M, et al. Authentication of anglerfish species (Lophius spp) by means of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and forensically informative nucleotide sequencing (FINS) methodologies [J]. J Agric Food Chem, 2008, 56(22): 10594-10599.
[5] MENDONCA F F, HASHIMOTO D T, PORTO-FORESTI F, et al. Identification of the shark species Rhizoprionodon lalandii and R. porosus (Elasmobranchii, Carcharhinidae) by multiplex PCR and PCR-RFLP techniques[J]. Mol Ecol Resour, 2009, 9(3): 771-773.
[6] ARANISHI F. Rapid PCR-RFLP method for discrimination of imported and domestic mackerel [J]. Mar Biotechnol, 2005, 7(6): 571-575.
[7] HSIEH H S, CHAI T J, HWANG D F. Using the PCR-RFLP method to identify the species of different processed products of billfish meats [J]. Food Control, 2007, 18(4): 369-374.
[8] NAGASE M, MAETA K, AIMI T, et al. Authentication of flying-fish-meal content of processed food using PCR-RFLP [J]. Fisheries Sci, 2009, 75(3): 811-816.
[9] ARANISHI F, OKIMOTO T, IZUMI S. Identification of gadoid species (Pisces, Gadidae) by PCR-RFLP analysis. J Appl Genet, 2005, 46(1): 69-73.
[10] CHEN T Y, HWANG D F. Electrophoretic identification of muscle proteins in 7 puffer species [J]. J Food Sci, 2002, 67(3): 936-942.
[11] CHEN T Y, SHIAU C Y, NOGUCHIA T, et al. Identification of puffer fish species by native isoelectric focusing technique [J]. Food Chem, 2003, 83(3): 475-479.
[12] CHEN T Y, SHIAU C Y, WEI C I, et al. Preliminary study on puffer fish proteome species identification of puffer fish by two-dimensional electrophoresis [J]. J Agric Food Chem, 2004, 52 (8): 2236–2241.
[13] ISHIZAKI S, YOKOYAMA Y, OSHIRO N, et al. Molecular identification of pufferfish species using PCR amplification and restriction analysis of a segment of the 16S rRNA gene [J]. Comp Biochem Physiol PT D, 2006, 1(1): 139-144.
[14] 陈文炳, 赵晨, 邵碧英等. PCR方法检测河豚鱼的引物筛选及反应体系优化 [J]. 食品科学, 2010, 31(20): 376-381.
[15] DOOLEY J J, SAGE H D, BROWN H M, et al. Improved fish species identification by use of lab-on-a-chip technology [J]. Food Control, 2005, 16(7): 601-607.
[16] 陈双雅, 张津, 陈伟玲等. 聚合酶链式反应-限制性片段长度多态性和芯片生物分析技术用于台湾海峡常见石斑鱼和鲷鱼的品种鉴定. 色谱, 2011, 29(7): 677-680.
[17] DOOLEY J J, CLARK M A, SAGE H D, et al. Fish species identification using PCR-RFLP analysis and lab-on-a-chip capillary electrophoresis: application to detect white fish species in food products and an inter-laboratory study [J]. J Agric Food Chem, 2005, 53 (9): 3348–3357.