Ultraviolet-Induced Mutagenesis of Cordyceps sinensis and Cordyceps militaris Protoplast Fusant

doi:10.7506/spkx1002-6630-201505023

Abstract

Abstract:

Protoplast fusants between Cordyceps sinensis and Cordyceps militaris were mutagenized by ultraviolet (UV)
irradiation for improved production of cordycepic acid and cordycepin in Cordyceps mycelia. Two mutant strains (Y-90
and YP-42) were selected showing a 1.80- and 1.33-fold increase in the production of cordycepic acid and cordycepin when
compared with the original funsant strain, respectively. Random amplified polymorphic DNA (RAPD) markers and ribosomal
DNA internal transcribed spacer (rDNA-ITS) sequence analysis were used to analyze the genetic variability. The ITS sequence
comparison revealed no genetic change after mutagenesis. RAPD analysis showed that the amplified fragments from the two
mutants were different from the parent strain, and the acquired characters of the mutants were caused by gene mutation.

Key words: Cordyceps sinensis, Cordyceps militaris, mutagenesis, cordycepic acid, cordycepin, fruit body, random amplified polymorphic DNA (RAPD), rDNA-ITS

CLC Number:

Q949.3

QU Hongyan, GUO Chengjin*. Ultraviolet-Induced Mutagenesis of Cordyceps sinensis and Cordyceps militaris Protoplast Fusant[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201505023.

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Ultraviolet-Induced Mutagenesis of Cordyceps sinensis and Cordyceps militaris Protoplast Fusant

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