FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (12): 97-100.

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Purification of Lipoxygenase from Tomato Fruit and Preparation of 9-hydroperoxides of Linoleic Acid

 WANG  Hui-Long, SUN  Yong-Feng, CHEN  Shu-Ling, ZHU  Su-Min, LUO  Chang-Rong   

  1. Huabao Food Flavor and Fragrance(Shanghai) Limited Company
  • Online:2005-12-15 Published:2011-10-24

Abstract: Lipoxygenase was extracted from tomato fruits,and the optimal extraction conditions for lipoxygenase activity were discussed. Then lipoxygenase was purified by ammonium sulphate fractionation. The results showed that lipoxygenase was purified 3.4-fold,and the recovery rate of lipoxygenase activity was 84%. Then 9-hydroperoxides from linoleic acid were prepared by the purified tomato lipoxygenase. The product was detected by reversed-phase HPLC with diode array detector. The results showed that the method was easy to track analysis of hydroperoxides of lipoxygenase products formed from linoleic acid in short time.

Key words: lipoxygenase, tomato, linoleic acid, HPLC