Study on Optimization of Extraction Conditions of Platycodon grandiflorum A. DC Polysaccharide Using Response Surface Methodology

Abstract

Abstract: Platycodon grandiflorum A. DC.(PADC) root was taken as experiment material to extract polysaccharide. On the base of single factor tests(extraction time,extraction temperature,liquid-solid ratio,solvent pH value and NaCl concentration) ,the study concluded the main factors affecting the extraction of PADC polysaccharide,which are liquid-solid ratio,extraction time and extraction temperature. Then through central-composite test design,the extraction conditions were concluded as liquid-solid ratio 34.43,extraction time 89.83 min and extraction temperature 52.47 ℃. By means of validation experiments,the adequacy of this model was confirmed.

Key words: response surface methodology, Platycodon grandiflorum A. DC, polysaccharide, extraction conditions, optimization

RUAN Zheng, ZHOU Quan-Cheng, DENG Ze-Yuan, YIN Yu-Long. Study on Optimization of Extraction Conditions of Platycodon grandiflorum A. DC Polysaccharide Using Response Surface Methodology[J]. FOOD SCIENCE, 2008, 29(12): 283-287.

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Study on Optimization of Extraction Conditions of Platycodon grandiflorum A. DC Polysaccharide Using Response Surface Methodology

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