FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (12): 555-558.

Previous Articles     Next Articles

Study on Phenol-Sulfuric Acid Method for Determination of Polysaccharide Content in Pleurotus eryngii

GUO  Jin-Long, CHEN  You-Jun, SUN  Guo-Qin, GUO  Jiu-Feng, WANG  Yun-Hua, ZHU  Xiao-Qing, NIE  Qi-Sen   

  1. 1.College of Biological Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China; 2.Biotechnology Research Center,Inner Mongolia Academy of Agricultural Sciences,Hohhot 010031,China; 3.Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China
  • Online:2008-12-15 Published:2011-12-08

Abstract: The factors affacting the determination of polysaccharide content in Pleurotus eryngii with phenol-sulfuric acid method were optimized. Results showed that under the conditions of detection wavelength 485 nm,phenol content 1.4 ml,reaction temperature 40 ℃ and reaction time 15 min,there is good linearity range from 10.00 to 70.00μg/ml(r=0.9996). The average recovery is 99.2% and the RSD is 1.16%.

Key words: Peurotus eryngii, polysaccharide, phenol-sulfuric acid method