Abstract:

The effect of Oudemansiella radicate polysaccharide (ORP) on the intestinal microbiota in Kunming male mice
was studied by Illumina high-throughput sequencing in this investigation. The mouse model of intestinal dysbiosis was
established by intragastric administration of lincomycin hydrochloride. Sixty mice were randomly divided into five groups
including normal control group, natural recovery group and three groups treated with Oudemansiella radicate polysaccharide
at different doses. Cecal contents were collected aseptically, and the intestinal microbiota was detected by illumina highthroughput
sequencing. The results showed that the diversity and abundance of intestinal bacteria were varied in the mice
in the five groups. Among these, the normal control group had the highest intestinal microbiota diversity followed by the
ORP treatment groups, and the intestinal microbiota of the natural recovery group exhibited the lowest species diversity. In
addition, the proportions of beneficial and pathogenic bacteria in the mouse intestine differed significantly among the five
groups; the proportion of beneficial bacteria was higher in the three treatment groups than in the two other groups and the
highest proportion of pathogenic bacteria was observed in the natural recovery group. indicating that Oudemansiella radicate
polysaccharide could improve and recover the intestinal microbiota of mice with intestinal dysbiosis.

Key words: Oudemansiella radicate, polysaccharide, intestinal microbiota, high-throughput sequencing