Abstract: The purpose of this study was to develop a macroporous resin adsorption method for the decolorization of Ganoderma lucidum polysaccharides. Ganoderma lucidum polysaccharides with a molecular weight between 10 kD and 500 kD were obtained by extracting Ganoderma lucidum fruiting bodies twice with boiling water, concentrating and ultrafiltrating with different MWCO (10 kD and 500 kD) membranes. Ten types of macroporous resin were compared for their effectiveness in decolorizing the polysaccharides. Based on decolorization rate and polysaccharide retention rate, type D303 macroporous resin had the best decolorization performance. Process parameter optimization for the decolorization of Ganoderma lucidum polysaccharides by static adsorption of type D303 macroporous resin were performed using one-factor-at-a-time combined with orthogonal array design method. The optimum conditions for static decoloration of resin D303 were as follows: sample concentration 15 mg/mL, pH 6, decoloration temperature 50 ℃, and decoloration time 7 h. Under the optimal conditions, a decoloration rate of 91.89% and a polysaccharide retention rate of 75.28% were obtained. The optimum conditions for dynamic decoloration of resin D303 were as follows: flow rate 3 BV/h (1 BV=10 mL), and sample loading amount 150 mg/mL resin, resulting in a decoloration rate of 92.01% and a polysaccharide retention rate of 71.85%. These findings collectively indicated that the resin D303 was more suitable for the decoloration of Ganoderma lucidum polysaccharides.

Key words: Ganoderma lucidum, polysaccharide, decoloration, resin