Orthogonal Array Design for Optimization of Extraction of Biochanin A from Chickpea Seed

doi:10.7506/spkx1002-6630-201006028

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (6): 127-130.doi: 10.7506/spkx1002-6630-201006028

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Orthogonal Array Design for Optimization of Extraction of Biochanin A from Chickpea Seed

TIAN Chun-yuan

School of Life Science and Technology, Xiaogan University, Xiaogan 432000, China

Received:2009-03-02 Online:2010-03-15 Published:2010-12-29
Contact: TIAN Chun-yuan E-mail:hbtcy@yahoo.com.cn

Abstract

Abstract:

Orthogonal array design was used to optimize hot ethanol reflux extraction of biochanin A from chickpea (Cicer arietinium L.) seed in this study. Results showed that an optimal compromise between biochanin A yield and purity was achieved by two-time extraction at a material/liquid ratio of 1:10 for 1.5 h each time. This extraction method, thanks to its convenience, safety, low cost and high efficiency, can be popularized for the large-scale production. Moreover, based on optimized hot ethanol reflux extraction, a RP-HPLC method was developed for rapid and accurate determination of biochanin A from chickpea seed.

Key words: chickpea (Cicer arietinium L.) seed, biochanin A, orthogonal array design, RP-HPLC

CLC Number:

TIAN Chun-yuan. Orthogonal Array Design for Optimization of Extraction of Biochanin A from Chickpea Seed[J]. FOOD SCIENCE, 2010, 31(6): 127-130.

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Orthogonal Array Design for Optimization of Extraction of Biochanin A from Chickpea Seed

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