基于膜滤法处理预发酵椰子水促进细菌纤维素的合成

摘要/Abstract

摘要： ：研究预发酵椰子水（Fermented Coconut Water，FCW）中促进细菌纤维素（Bacterial Cellulose，BC）合成的原因，采用膜过滤法处理FCW得到膜滤上清液和菌体，接种驹形氏杆菌（Komagataeibacter nataicola）Y19至培养基中30℃静置培养7d。结果显示，上清液组的BC产量达到5.65g/L，比对照组新鲜椰子水（Nature Coconut Water，NCW）提高10倍，而菌体沉淀组对BC产量的促进显著弱于上清液组和FCW组，说明膜滤上清液对BC合成的促进作用显著。通过对膜滤上清液进行气相色谱-质谱法（GC-MS）分析以及利用高效液相色谱法（HPLC）对预发酵过程中有机酸的变化分析，发现上清液中能检出的挥发性成分总量约为NCW的5倍，醇类相对含量最高，约占62 %，其中乙醇和乙酸含量分别比NCW提高了4.5倍和48倍。预发酵过程中，乙酸和乳酸的含量逐渐增加，经过1-2d预发酵后葡萄糖酸含量显著降低，其他有机酸在预发酵过程中变化不显著。结果显示椰子水预发酵过程中酵母菌、乳酸菌及醋酸菌等微生物对后期BC合成起到重要作用，且椰子水经预发酵后变化较大的醇类和酸类物质通过作为碳源以及参与K. nataicola Y19的代谢调节而影响BC的合成。

关键词: 膜过滤, 预发酵椰子水, 细菌纤维素, 促进作用

Abstract: To study the reason for promoting the synthesis of bacterial cellulose (Bacterial Cellulose, BC) in Fermented Coconut Water (FCW). The membrane filtration supernatant was treated by membrane filtration to obtain membrane filtration supernatant and bacteria, and inoculated with (Komagataeibacter nataicola) Y19 was cultured in the medium at 30℃ for 7 days. The results showed that the BC yield of the supernatant group reached 5.65 g / L, which was 10 times higher than that of the control group, and the BC yield was significantly weaker than that of the supernatant group. The FCW group indicated that the membrane filtration supernatant promoted the BC synthesis significantly. Gas chromatography-mass spectrometry (GC-MS) analysis of membrane filtration supernatant and analysis of changes in organic acids during pre-fermentation by high performance liquid chromatography (HPLC) revealed volatiles detected in the supernatant. The total amount of sexual components is about 5 times that of NCW, and the relative content of alcohols is the highest, accounting for 62%. The content of ethanol and acetic acid is 4.5 times and 48 times higher than that of NCW, respectively. During the pre-fermentation process, the content of acetic acid and lactic acid increased gradually. After 1-2d pre-fermentation, the gluconic acid content decreased significantly, and other organic acids did not change significantly during the pre-fermentation process. The results showed that yeast, lactic acid bacteria and acetic acid bacteria played an important role in the synthesis of BC in the process of coconut water pre-fermentation, and the alcohols and acids that changed greatly after pre-fermentation affected the synthesis of BC by serving as carbon source and participating in the metabolic regulation of K. nataicola Y19.

Key words: membrane filtration, pre-fermented coconut water, bacterial cellulose, promoting mechanism

中图分类号:

TS201.3

傅美娟刘四新. 基于膜滤法处理预发酵椰子水促进细菌纤维素的合成[J]. 食品科学, 0, (): 0-0.

FU MeiJuan. Promoting Bacterial Cellulose Synthesis by Pre-fermented Coconut Water Based on Membrane Filtration[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]Mazhar Ul-Islam,Taous Khan,Joong Kon Park. . Water holding and release properties of bacterial cellulose obtained by in situ and ex situ modification[J]. Carbohydrate Polymers,, 2012, 88(2):596-603
[2] 刘四新，李枚秋，方仲根.椰子纳塔发酵条件研究[J].食品与发酵工业, 1999, 25(1):36-39
[3] Campano, C., Balea, A., Blanco, A., et al. Enhancement of the fermentation process and properties of bacterial cellulose: a review[J]. Cellulose, 2016, 23(1):57-91
[4] Wang, S., Jiang, F., Xu, X., et al. Super-strong, superstiffff macrofifibers with aligned, long bacterial cellulose Nanofibers[J].Bacterial Nanofibers,Adv.Mater., 2017, :1-8
[5]Zhu C，Li F，Zhou X，et al.Kombucha-synthesized bacterial cellulose：preparation，characterization，and biocompatibility evaluatio[J].Journal of Biomedical Materials Research(Part A), 2014, 102(5):1548-1557
[6]Cheng K C，Catchmark J M，Demirci A.Enhanced production of bacterial cellulose by using a biofilm reactor and its material property analysis[J].Journal of Biological Engineering, 2009, 3(12):1-10
[7]Agoda-Tandjawa G，Durand S，Gaillard C，et al.Properties of cellulosepectins composites：implication for structural and mechanical properties of cell wall[J].Carbohydr Polym, 2012, 90(2):1081-1091
[8]Santos S M， Carbajo J M， Quintana E， et al.Characterization of purified bacterial cellulose focused on its use on paper restoration[J].Carbohydrate Polymers, 2015, 116(2):173-81
[9] 孙勇慧，刘鹏涛，刘忠.细菌纤维素的应用进展[J].材料导报, 2015, 29(05):62-67
[10] Shah, N., Ul-Islam, M., Khattak, W.A., et al. Overview of bacterial cellulose composites: a multipurpose advanced material[J].Carbohydrate Polymers, 2013, 98(2):1585-1598
[11]Jianbin Ye, Shanshan Zheng, Zhan Zhang, et al.Bacterial cellulose production by Acetobacter xylinum ATCC 23767 using tobacco waste extract as culture medium[J].Bioresource Technology, 2019, 274(2019):518-524
[12] Cynthia Radiman, Galuh Yuliani.Coconut water as a potential resource for cellulose acetate membrane preparation[J].Polymer International, 2008, 57(3):502-508
[13] Jahan, F., Kumar, V., Saxena, R.K., . Distillery effluent as a potential medium for bacterial cellulose production: a biopolymer of great commercial importance[J].Bioresource Technology, 2018, 250:922-926
[14] Costa Andrea F S, Almeida Fabíola C G, Vinhas Glória M, et al.Production of Bacterial Cellulose by Gluconacetobacter hansenii Using Corn Steep Liquor As Nutrient Sources[J].Frontiers in microbiology, 2017, 8:20-27
[15]S.S. Kim,SY. Lee,K.J. Park,et al.Gluconacetobacter sp. gel_SEA623-2,bacterial cellulose producing bacterium isolated from citrus fruit juice[J].Saudi Journal of Biological Sciences, 2015, 24(2):314-319
[16]Huang, C.Yang,XY.,Xiong,L.,et al. Utilization of corncob acid hydrolysate for bacterial cellulose production by Gluconacetobacter xylinus[J].Applied Biochemistry and Biotechnology, 2014, 175(3):1678-1688
[17]邓健, 刘四新, 杨一冲等.椰子水的发酵预处理对细菌纤维素合成促进作用研究[J].广东农业科学, 2015, 42(19):84-88
[18]王艳梅, 贾佳, 胡淇淞等.椰子水的自然预发酵条件对细菌纤维素合成的促进性影响[J].热带作物学报, 2018, 39(1):151-155
[19] Zhang J, Yang Y, Deng J, et al.Dynamic profile of the microbiota during coconut water pre-fermentation for nata de coco production[J].LWT-Food Science and Technology, 2017, 81:87-93
[20] 杨一冲，椰纤果生产中自然发酵椰子水的菌相变化研究[D].海口:海南大学, 2015:24-40.
[21]Bi J C，Liu S X，Li C F，et al.Morphology and structure characterization of bacterial celluloses produced by different strains in agitated culture[J].Journal of Applied Microbiology, 2014, 117(5):1305-1311
[22] Li Y, Wang S, Huang R, et al.Improvement of bacterial cellulose production by manipulating the metabolic pathways in which ethanol and sodium citrate involved[J].Applied Microbiology and Biotechnology, 2012, 96(6):1479-1487
[23]Sakurai K, Arai H, Ishii M, et al.Changes in the gene expression profile of Acetobacter aceti during growth on ethanol[J].Journal of bioscience and bioengineering, 2012, 113(3):343-348
[24] Mohammadkazemi F, Azin M, Ashori A.Production of bacterial cellulose using different carbon sources and culture media[J].Carbohydrate Polymers, 2015, 117:518-523
[25]张丽平, 卢红梅, 戴锐等.乙醇及有机酸对木醋杆菌合成细菌纤维素的影响[J].食品工业科技, 2014, 35(4):161-165
[26]马霞, 贾士儒, 关凤梅等.有机酸对木醋杆菌合成细菌纤维素的影响规律[J].纤维素科学与技术, 2003, 11(1):34-37
[27] Takaaki Naritomi, Tohru Kouda, Hisato Yano, et al.Effect of lactate on bacterial cellulose production from fructose in continuous culture[J].Journal of Fermentation and Bioengineering, 1998, 85(1):598-603
[28]Xiao-Yan Yang, Chao Huang, Hai-jun Guo, et al.Beneficial Effect of Acetic Acid on the Xylose Utilization and Bacterial Cellulose Production by Gluconacetobacter xylinus[J].Indian Journal of Microbiology, 2014, 54(3):268-273
[29]Zhong C，Zhang G C，Liu M，et al.Metabolic flux analysis of Gluconacetobacter xylinus for bacterial cellulose production[J].Applied Microbiology and Biotechnology, 2013, 97(14):6189-6199
[30]李欣，颜彩玲，潘凌鸿，等.细菌纤维素的合成与调控进展[J].生物加工过程, 2011, 9(4):64-68