Optimization of Extraction of Polysaccharides from Trichoderma pseudokoningii Mycelium

doi:10.7506/spkx1002-6630-201310008

Abstract

Abstract:

Objective: To study the optimal extraction conditions of polysaccharides from Trichoderma pseudokoningii mycelium.
Methods: One-factor-at-a-time experiments and orthogonal array design were applied to establish the optimum extraction
conditions based on the yield of polysaccharide. Results: The influence of three factors on extraction efficiency decreased in the
following order: reflux temperature ＞ extraction number ＞extraction time ＞ particle size. The optimal extraction conditions
were obtained as follows: three extraction cycles at 100 ℃ for 2 h each with a solid/liquid ratio of 1:30 (g/mL). Under these
conditions, the yield of polysaccharides from Trichoderma pseudokoningii was (11.09 ± 0.13)%. Conclusion: The optimized
process is stable, feasible and applicable for the extraction of polysaccharides from Trichoderma pseudokoningii mycelium.

Key words: Trichiderma seudokoningii, polysaccharide, orthogonal array design experiments

CLC Number:

R939.9

LI Ping，QIN Guo-zheng，WANG Guo-dong，CHEN Kao-shan，CHEN Fu-hua. Optimization of Extraction of Polysaccharides from Trichoderma pseudokoningii Mycelium[J]. FOOD SCIENCE, 2013, 34(10): 35-38.

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