关键词: 牛蒡, 菊糖, 提取, 纯化

Abstract:

Crude inulin was obtained from dried burdock root powder by water extraction followed by ethanol precipitation and deproteinized by combination of papain hydrolysis and Sevag method or each of them. On the basis of single factor experiments in which the effects of material/liquid ratio, extraction time, temperature and extraction times on yield of inulin were studied, an orthogonal array design involving 9 experiments of other three factors except extraction temperature was adopted to maximize yield of inulin. The twice extraction using 10-fold volume of water for 120 min at 80 ℃ gave a maximum yield of inulin of 63.36%. The combination papain hydrolysis and Sevag method exhibited higher protein removal efficiency (90.83％) than each of them.

Key words: burdock, inulin, extraction, purification