食品科学 ›› 2009, Vol. 30 ›› Issue (20): 308-311.doi: 10.7506/spkx1002-6300-200920067

• 分析检测 • 上一篇    下一篇

中华绒螯蟹蟹肉挥发性风味成分分析

蒋根栋,陈舜胜   

  1. 上海海洋大学食品学院
  • 收稿日期:2008-09-08 出版日期:2009-10-15 发布日期:2010-12-29
  • 通讯作者: 陈舜胜 E-mail:sschen@shou.edu.cn

Volatile Flavor Components in Meat of Chinese Mitten Crab (Eriocheir sinensis)

CHEN Shun-sheng,JIANG Gen-dong   

  1. College of Food Science, Shanghai Ocean University, Shanghai 200090, China
  • Received:2008-09-08 Online:2009-10-15 Published:2010-12-29
  • Contact: CHEN Shun-sheng E-mail:sschen@shou.edu.cn

摘要:

采用顶空固相微萃取(HS-SPME)方法提取中华绒螯蟹蟹肉中的挥发性风味成分,利用气相色谱-质谱法(GC-MS)对提取的风味成分进行分离鉴别。结果表明,萃取温度60℃、时间50min、NaCl添加量0.16g/ml的条件下,挥发性风味成分的吸附效果最好。中华绒螯蟹的蟹肉中鉴别出40种挥发性成分,其中醛类8种、酮类3种、醇类3种、酯类1种、烷烃类13种、芳香类化合物4种、含氮化合物4种、其他化合物4种。中华绒螯蟹的蟹肉中,三甲胺(TMA)、壬醛和十四烷的含量较高,其含量分别达到挥发性成分总含量的20%、18%和10%左右。

关键词: 中华绒螯蟹, 风味, 固相微萃取(SPME), 气相色谱-质谱法(GC-MS)

Abstract:

Headspace-solid phase micro-extraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) was adopted to analyze volatile flavor components in meat of Chinese mitten crab. The optimum extraction efficiency expressed as peak area in GC-MS chromatogram was achieved through the extraction for 50 min at 60 ℃ with the amount of added NaCl of 0.16 g/ml. Totally 40 compounds were identified, including 8 aldehydes, 3 ketones, 3 alcohols, 13 hydrocarbons, 1 ester, 4 aromatic compounds, 4 nitrogen-containing compounds and 4 other compounds. Trimethylamine (TMA), nonanal and tetradecane were main volatile compounds in crabmeat, which accounted for 20%, 18% and 10% of total volatile compounds, respectively.

Key words: Chinese mitten crab, flavor, solid phase micro-extraction (SPME), gas chromatography-mass spectrometry (GC-MS)

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