关键词: 甜味剂, 乙酰磺胺酸钾, 糖精钠, 甜蜜素, 阿斯巴甜, 阿力甜, 高效液相色谱

Abstract:

To establish a rapid analysis method for determination of common sweeteners such as acesulfame potassium, saccharin sodium, sodium cyclamate, aspavtame, and alitame in milk beverage by HPLC. After the pretreatments of dilution, protein precipitation, and high-speed centrifugation, the sample was separated by Symmetry C18 column with phosphate buffer (pH 5.0) and acetonitrile as mobile phase, eluted gradiently, and detected at the UV wavelength of 205 nm. The analysis of one sample costs 12 min, and all five sweeteners could be well separated. The average spike recoveries of the above five sweeteners were 86.0% to 102%, and the RSDs were 2.1% to 5.6% (n = 6). This method is simple, quick, and of good reproducibility and selectivity, therefore it is an effective way to determine sweeteners in milk drink.

Key words: sweetener, acesulfame potassium, saccharin sodium, sodium cyclamate, aspavtame, alitame, HPLC