固定化分枝杆菌BD696－6产雄烯二酮发酵条件的研究

doi:10.7506/spkx1002-6630-200915039

食品科学 ›› 2009, Vol. 30 ›› Issue (15): 172-175.doi: 10.7506/spkx1002-6630-200915039

固定化分枝杆菌BD696－6产雄烯二酮发酵条件的研究

冯丽桦¹，潘丽军¹，姜绍通¹，杨英^{1 , 2}，罗炳华¹

1.合肥工业大学生物与食品工程学院 2.安徽建筑工业学院环境工程系

收稿日期:2008-10-20 修回日期:2009-06-17 出版日期:2009-08-01 发布日期:2010-12-29
通讯作者: 潘丽军 E-mail:panlijun1955@163.com
基金资助:
“十一五”国家科技支撑计划项目(2006BAD05A12)；安徽省自然科学基金项目(070413265X)

Fermentation of Immobilized Mycobacterium sp. BD 696-6 for Production of Androst-4-ene-3,17-dione from Rapeseed Sterols

FENG Li-hua1，PAN Li-jun1，JIANG Shao-tong1， YANG Ying1,2，LUO Bing-hua1

1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China；
2. Department of Environmental Engineering，Anhui Institute of Architecture, Hefei 230022, China

Received:2008-10-20 Revised:2009-06-17 Online:2009-08-01 Published:2010-12-29
Contact: PAN Li-jun1， E-mail:panlijun1955@163.com

摘要/Abstract

摘要：

利用海藻酸钙凝胶颗粒将分枝杆菌BD 696-6 固定，进行固定化细胞的发酵条件研究。实验结果表明：发酵最适条件为：pH8.5、温度32℃、底物浓度0.5%、转化时间144h，较游离细胞发酵周期缩短了24h，雄烯二酮产量明显提高，得率均在78% 以上，且固定化细胞的贮藏稳定性、重复使用效果较好。电镜扫描结果显示以海藻酸钙为包埋载体时，营养物质可自由传输。

关键词: 细胞固定化, 雄烯二酮, 甾醇, 海藻酸钙, 分枝杆菌

Abstract:

Mycobacterium sp.BD 696-6, a mutant strain of Mycobacterium sp.BD 696-6 that can effectively convert sterols into androst-4-ene-3,17-dione (AD), was immobilized by entrapment in calcium alginate gel capsules. AD was produced from rapeseed sterols by the fermentation of immobilized Mycobacterium sp.BD 696-6. The effects of initial pH of fermentation medium, temperature, substrate concentration and fermentation time on AD yield were investigated by sing-factor method. In addition, the storage stability and repeated usability of immobilized Mycobacterium sp.BD 696-6 were evaluated and gel capsules embedding Mycobacterium sp.BD 696-6 cells after fermentation were observed under scanning electron microscopy. An AD yield of more than 78% was obtained by the fermentation under substrate concentration 0.5%, initial pH 8.5 and temperature 32 ℃ for 144 h which was shortened by 24 h when compared with the fermentation time of free cell. Immobilized Mycobacterium sp. BD696-6 showed excellent storage stability and after used repeatedly 9 times its mechanical strength and AD conversion capacity had no marked decrease. SEM images revealed that the nutrients for calcium alginate-immobilized Mycobacterium sp. BD 696-6 could be freely transported.

Key words: immobilized cells, androst-4-ene-3,17-dione, sterols, calcium alginate, Mycobacterium sp.

中图分类号:

Q949.3

冯丽桦1，潘丽军1，姜绍通1，杨英1,2，罗炳华1. 固定化分枝杆菌BD696－6产雄烯二酮发酵条件的研究[J]. 食品科学, 2009, 30(15): 172-175.

FENG Li-hua1，PAN Li-jun1，JIANG Shao-tong1， YANG Ying1,2，LUO Bing-hua1. Fermentation of Immobilized Mycobacterium sp. BD 696-6 for Production of Androst-4-ene-3,17-dione from Rapeseed Sterols[J]. FOOD SCIENCE, 2009, 30(15): 172-175.

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固定化分枝杆菌BD696－6产雄烯二酮发酵条件的研究

Fermentation of Immobilized Mycobacterium sp. BD 696-6 for Production of Androst-4-ene-3,17-dione from Rapeseed Sterols

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