食品科学 ›› 2010, Vol. 31 ›› Issue (6 ): 148-150.doi: 10.7506/spkx1002-6630-201006033

• 分析检测 • 上一篇    下一篇

电子鼻分析猪肉中负载的微生物数量研究

王丹凤,王锡昌,刘 源* ,赵 勇,顾赛麒,谢 晶,潘迎捷   

  1. 上海海洋大学食品学院
  • 收稿日期:2009-08-20 出版日期:2010-03-15 发布日期:2010-12-29
  • 通讯作者: 刘 源 E-mail:yliu@shou.edu.cn
  • 基金资助:

    国家“863”计划项目(2008AA100804);上海市科委重大科技攻关项目(07dz19508);
    上海市科委启明星计划(07QA14047)

Estimation of Total Bacterial Count in Pork Using Electronic Nose

WANG Dan-feng,WANG Xi-chang,LIU Yuan*,ZHAO Yong,GU Sai-qi,XIE Jing,PAN Ying-jie   

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Received:2009-08-20 Online:2010-03-15 Published:2010-12-29
  • Contact: LIU Yuan E-mail:yliu@shou.edu.cn

摘要:

利用电子鼻技术检测猪肉在4℃和20℃保存不同天数的挥发性成分的变化,结合猪肉中微生物数量的变化,考察电子鼻输出信号与微生物数量之间的对应关系。结果表明:主成分分析(PCA)可以区分不同储藏天数的猪肉样品;通过最小线性回归分析(PLS)建立电子鼻输出信号与细菌总数之间的对应关系,4℃时R = 0.9003,20℃时R =0.9940,线性关系均良好。初步研究表明,随着保存时间不同,猪肉挥发性成分与微生物数量会发生变化,电子鼻可检测到这些变化,因此可尝试用电子鼻技术检测猪肉中的有害微生物。

关键词: 猪肉, 电子鼻, 微生物, 检测

Abstract:

To provide experimental basis for estimation of total bacterial count in pork using electronic nose, mathematical models describing the relationship between output signal of electronic nose indicating change in volatile composition of chilled pork stored at 4 or 20 ℃ and total bacterial count in pork were investigated by partial least squares regression (PLS-regression). Results showed that pork samples stored for different days could be discriminated by principal component analysis (PCA). The correlation coefficients of developed mathematical models for estimating total bacterial count in pork stored at 4 or 20 ℃ were 0.9003 and 0.9940, respectively, which revealed good linearity. Electronic nose can accurately detect the changes in volatile composition and total bacterial count in pork during storage, thereby providing a useful tool for detecting harmful microorganisms in pork.

Key words: pork, electronic nose, microorganism, detection

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