食品科学 ›› 2010, Vol. 31 ›› Issue (10): 42-46.doi: 10.7506/spkx1002-6630-201010009

• 工艺技术 • 上一篇    下一篇

微波协同酶法提取玉米须多糖工艺的优化研究

陈 红,张艳荣,王大为* ,刘婷婷,张 波   

  1. 吉林农业大学食品科学与工程学院
  • 收稿日期:2009-08-03 修回日期:2009-12-01 出版日期:2010-05-15 发布日期:2010-12-29
  • 通讯作者: 王大为 E-mail:chenhong216@yahoo.com.cn
  • 基金资助:

    “十一五”国家科技支撑计划项目(2006BAD27B07)

Microwave-assisted Enzymatic Hydrolysis of Corn Silk for Extraction of Polysaccharides

CHEN Hong,ZHANG Yan-rong,WANG Da-wei*,LIU Ting-ting,ZHANG Bo   

  1. College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China
  • Received:2009-08-03 Revised:2009-12-01 Online:2010-05-15 Published:2010-12-29
  • Contact: WANG Da-wei E-mail:chenhong216@yahoo.com.cn

摘要:

采用微波协同酶法提取玉米须多糖并对其工艺进行优化。通过单因素试验和正交试验,确定微波协同酶法提取的最佳工艺条件:微波功率500W、微波处理时间2min、液料比30:1(mL/g)、纤维素酶用量1.5%、酶解温度50℃、酶解时间40min、pH5.0,在此工艺条件下,多糖提取率为8.12%。

关键词: 玉米须, 多糖, 提取, 微波, 纤维素酶

Abstract:

The present study aimed at developing an optimal procedure for the extraction of polysaccharides from corn silk hydrolyzed with a commercial cellulase preparation under microwave assistance. The optimal values of crucial technological parameters for improved polysaccharides yield were determined by one-factor-at-a-time and orthogonal array design methods as follows: microwave powder 500 W for 2 min treatment, water/material ratio 30:1 (mL/g), enzyme dosage 1.5%, pH 5.0 and hydrolysis temperature 50 ℃ for a hydrolysis duration of 40 min. Under such conditions, a maximum polysaccharides yield of up to 8.12% was obtained.

Key words: corn silk, polysaccharides, extraction, microwave, cellulase

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