食品科学 ›› 2011, Vol. 32 ›› Issue (20): 95-98.doi: 10.7506/spkx1002-6630-201120020

• 工艺技术 • 上一篇    下一篇

苹果多酚的提取及抗氧化活性研究

郭娇娇1,方 敏2,林利美1,宫智勇1,*   

  1. 1.武汉工业学院食品科学与工程学院 2.华中科技大学同济医学院公共卫生学院营养与食品卫生系
  • 出版日期:2011-10-25 发布日期:2011-10-12
  • 基金资助:
    国家“863”计划项目(2010AA023003)

Extraction and Antioxidant Properties of Polyphenols from Apple

GUO Jiao-jiao1,FANG Min2,LIN Li-mei1,GONG Zhi-yong1,*   

  1. (1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2. Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China)
  • Online:2011-10-25 Published:2011-10-12

摘要: 通过单因素及正交试验,对苹果多酚的提取工艺进行研究。确定苹果多酚的最佳提取工艺为乙醇体积分数30%、温度70℃、料液比1:6、提取时间45min,此时苹果多酚的含量为9.032mg/g。采用DPPH法和亚铁还原能力(ferric reducing ability of plasma,FRAP)法对苹果多酚的抗氧化活性进行研究,结果显示,它对DPPH自由基的清除率达到94.6%,此时其FRAP值为546.4μmol/L。

关键词: 苹果多酚, 抗氧化, DPPH自由基, FRAP

Abstract: One-factor-at-a-time coupled with orthogonal array design method was employed to optimize the extraction of polypehnols from apple. The optimal extraction conditions were determined as follows: 30% ethanol as the extraction solvent at a material/liquid ratio of 1:6, 70 ℃ temperature and 45 min extraction time. Under these conditions, the extraction yield of polyphenols from apple was 9.032 mg/g. The DPPH radical scavenging rate of apple polyphenols extracted was 94.6% and the FRAP 546.4 μmol/L.

Key words: apple polyphenols, antioxidant properties, DPPH free radicals, FRAP assay

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