食品科学 ›› 2011, Vol. 32 ›› Issue (21): 223-228.doi: 10.7506/spkx1002-6630-201121046

• 生物工程 • 上一篇    下一篇

降胆固醇乳酸菌的筛选及对大鼠血脂的影响

杨 琴1,胡 颖1,甘盛力1,朱秋劲1,2,3,*,何腊平1,2,3,胡 萍1,2,3,朱建荣3,4   

  1. 1.贵州大学生命科学院 2.贵州大学 食品科学工程研究中心 3.贵州省 乳品工程技术研究中心 4.贵阳三联乳业有限公司
  • 出版日期:2011-11-15 发布日期:2011-11-11
  • 基金资助:
    科技部农业技术成果转化项目(2009GB2F200329);贵阳市重大科技专项(筑科农合同字第2-017 号)

Screening of Cholesterol-reducing Lactic Acid Bacteria and Its Effect on Blood Lipids in Rats

YANG Qin1,HU Ying1,GAN Sheng-li1,ZHU Qiu-jin1,2,3,*,HE La-ping1,2,3,HU Ping1,2,3,ZHU Jian-rong3,4   

  1. (1. College of Life Sciences, Guizhou University, Guiyang 550025, China;2. Food Science and Engineering Research Center, Guizhou University, Guiyang 550025, China;3. Guizhou Province Dairy Engineering Technology Research Center, Guiyang 550018, China;4. Guiyang Sanlian Dairy and Food Co. Ltd., Guiyang 550018, China)
  • Online:2011-11-15 Published:2011-11-11

摘要: 对分离自发酵品及肠道排泄物的乳酸菌进行降胆固醇体外筛选及体内评价。结果显示:所分离得到的菌株中,对MRS-CHOL培养基胆固醇降解率最高的是R-2菌株(26.97±0.51)%和I-2菌株(23.72±0.41)%;R-2和I-2菌株对酸(pH3.0)有一定耐受性,在0.3g/100mL胆盐质量浓度条件下培养6h后,I-2的活菌数为1.1×104CFU/mL,R-2的活菌数为2.2×106CFU/mL。与对照组相比,采用I-2菌株和R-2菌株饲喂高脂大鼠,均可明显抑制大鼠总胆固醇(TC) (P<0.01) 和低密度脂蛋白胆固醇(LDL-C)(P<0.05)升高;经鉴定,I-2菌株和R-2菌株分别为海氏肠球菌(Enterococcus hirae)和屎肠球菌(Enterococcus faecium)。

关键词: 乳酸菌, 降胆固醇, 筛选, 发酵, 肠球菌属, 动物模型

Abstract: In this study, lactic acid bacteria with the ability to degrading cholesterol were screened from fermented products and human feces. Strains R-2 and I-2 had the highest cholesterol degradation rate in the MRS-CHOL culture medium among the selected strains, reaching( 26.97 ± 0.51)% and (23.72 ± 0.41)%, respectively. Both strains exhibited good tolerance to low pH (3.0). In the presence of 0.3 g/100 mL bile salt, the viable cell counts of I-2 and R-2 after 6 hours of cultivation were 1.1 × 104 CFU/mL and 2.2 × 106 CFU/mL, respectively. Moreover, R-2 and I-2 could inhibit the increase of TC (P < 0.01) and LDL-C (P <0.05) to some extent. R-2 and I-2 were identified as Enterococcus faecium and Enterococcus hirae, respectively by bacterial identification technology.

Key words: lactic acid bacteria, cholesterol-reducing, screening, fermentation, Eenterococcus, animal model

中图分类号: