食品科学 ›› 2012, Vol. 33 ›› Issue (7): 93-95.doi: 10.7506/spkx1002-6630-201207020

• 基础研究 • 上一篇    下一篇

芒果多糖的纯化与光谱分析

孙延芳1,梁宗锁2,张 欣3,Unger FRANK4   

  1. 1.辽宁工程技术大学理学院 2.西北农林科技大学生命科学学院 3.西北农林科技大学食品科学与工程学院 4.维也纳大学生物药理和药理技术实验室
  • 出版日期:2012-04-15 发布日期:2012-04-20
  • 基金资助:
    中国科学院知识创新工程项目(KZCX2-XB2-05-01);“十一五”国家科技支撑计划项目(2008BAD98B08)

Purification and Spectroscopic Analysis of Polysaccharide from Mango

SUN Yan-fang1,LIANG Zong-suo2,ZHANG Xin3,Unger FRANK4   

  1. (1. College of Science, Liaoning Technical University, Fuxin 123000, China;2. College of Life Science, Northwest A&F University, Yangling 712100, China;3. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China; 4. Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna A-1090, Austria)
  • Online:2012-04-15 Published:2012-04-20

摘要: 采用热水浸提、乙醇沉淀法得到芒果多糖粗品;采用苯酚-硫酸法测其多糖含量为(46.3±1.75)mg/g。DEAE-25纤维素和Sephadex G-75凝胶层析柱进一步分离纯化得到白色絮状多糖;高效阴离子交换色谱法分析其单糖组成。结果表明:该多糖是由鼠李糖、半乳糖和葡萄糖等单糖组成,其含量分别为(6.3±1.40)、(11.9±1.76)mg/g和(13.8±1.52)mg/g。红外光谱分析表明具有明显的多糖特征吸收峰,该多糖结构主要为β-糖苷键连接的吡喃型葡聚糖。

关键词: 墨西哥芒果, 多糖, 高效阴离子交换色谱, 红外光谱

Abstract: Crude polysaccharide was extracted from Mexico mango using hot water extraction followed by ethanol precipitation. The polysaccharide concentration of crude extract was (46.3±1.75) mg/g as determined by phenol-sulfuric acid assay. After further purification by DEAE-52 and Sephadex G-75 column chromatography, a white flocculent polysaccharide was obtained. High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) was used to determine the monosaccharide composition. The results showed that the polysaccharide was composed of rhamnose, galactose and glucose with a ratio of (6.3±1.40), (11.9 ±1.76) mg/g and (13.8 ±1.52) mg/g, respectively. The purified polysaccharide exhibited the characteristic IR absorption of polysaccharide. The structure was pyranoid glucan with β-glycosidic bond.

Key words: mango, polysaccharide, high performance anion exchange chromatography (HPAEC), infrared spectroscopy (IR)

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