代谢组学法评价紫苏子抗小鼠急性肝损伤的作用

doi:10.7506/spkx1002-6630-201417050

食品科学

代谢组学法评价紫苏子抗小鼠急性肝损伤的作用

耿芹，郑床木，管政，张小红，朱超，杨曙明，陈爱亮

1.华烁科技股份有限公司，湖北武汉 430074；2.中国农业科学院农业质量标准与检测技术研究所，北京 100081；
3.浙江中医药大学药物研究所，浙江杭州 310053

出版日期:2014-09-15 发布日期:2014-09-12

Protective Effect of Perilla Seed on Acute Hepatic Injury in Mice as Evaluated by Metabolomic Analysis

GENG Qin, ZHENG Chuang-mu, GUAN Zheng, ZHANG Xiao-hong, ZHU Chao, YANG Shu-ming, CHEN Ai-liang

1. Haiso Technology Co. Ltd., Wuhan 430074, China; 2. Institute of Quality Standards and Testing Technology for Agro-products,Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3. Institute of Materia Medica,Zhejiang Chinese Medical University, Hangzhou 310053, China

Online:2014-09-15 Published:2014-09-12

摘要/Abstract

摘要：

用代谢组学法对紫苏子抗小鼠急性肝损伤作用进行评价。研究构建四氯化碳（CCl4）致小鼠急性肝损伤模型，采用流动注射-三重四极杆-飞行时间质谱联用系统对模型组、紫苏子给药组、阳性药联苯双酯给药组及正常对照组动物的肝组织及全血代谢轮廓谱进行比较。采用主成分分析法对各组数据进行分析并初步筛选潜在生物标志物。代谢组学分析、血生化指标检测及病理研究表明，20 g/kg生药当量的紫苏子醇提物对CCl4致小鼠急性肝损伤模型具有与联苯双酯临床等效剂量相似的保肝作用。结果表明，柠檬酸、磷脂酸、溶血磷脂酰胆碱、溶血磷脂酰乙醇胺等9 个代谢物为CCl4致小鼠急性肝损伤及紫苏子保肝作用的潜在生物标志物；作用途径与脂质过氧化、能量代谢等有关。

关键词: 紫苏子, 流动注射, 质谱, 急性肝损伤, 代谢组学, 主成分分析

Abstract:

The objective of this study was to evaluate the protective effect of perilla (Perilla frutescens) seed on acute
hepatic injury induced by carbon tetrachloride. Identification of potential biomarkers was performed by high-performance
liquid chromatography (HPLC) and flow injection electrospray ionization with time-of-flight (TOF) mass spectrometry
(FIMS) combined with principal component analysis (PCA). Blood and hepatic tissue metabolic profiles were acquired
and correlated with the biochemical and histopathological results. In this work, metabolomic profiling, and biochemical
and histopathological studies together confirmed the liver protective effect of perilla seed. Perilla seed at a dose equivalent
to 20 g/kg per day caused a similar effect to that of bifendate (3.5 mg/kg per day). Compared with normal control group,
the expression of LysoPC, LysoPE, PA, carnitine, citric acid, inosine, methylated nucleoside, and taurohyocholate were
increased significantly in model group, whereas the opposite results were observed for the animals which had given perilla
seed extract. These findings indicated that the developed metabonomic method based on FIMS might be used as a potentially
powerful tool for fast and comprehensive estimation of the liver protective effect of perilla seeds.

Key words: perilla seed, flow injection, mass spectrometry, acute hepatic injury, metabonomics, principal component analysis

中图分类号:

TS255.1

耿芹，郑床木，管政，张小红，朱超，杨曙明，陈爱亮. 代谢组学法评价紫苏子抗小鼠急性肝损伤的作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417050.

GENG Qin, ZHENG Chuang-mu, GUAN Zheng, ZHANG Xiao-hong, ZHU Chao, YANG Shu-ming, CHEN Ai-liang. Protective Effect of Perilla Seed on Acute Hepatic Injury in Mice as Evaluated by Metabolomic Analysis[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417050.

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代谢组学法评价紫苏子抗小鼠急性肝损伤的作用

Protective Effect of Perilla Seed on Acute Hepatic Injury in Mice as Evaluated by Metabolomic Analysis

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