食品科学

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数码成像比色法测定牛乳中蛋白质的含量

乐 薇,刘 乐   

  1. 武汉工商学院环境与生物工程学院,湖北 武汉 430065
  • 出版日期:2016-02-25 发布日期:2016-02-23

Determination of the Protein Content of Milk by Digital Imaging Colorimetry

YUE Wei, LIU Le   

  1. College of Environmental and Biological Engineering, Wuhan Technology and Business University, Wuhan 430065, China
  • Online:2016-02-25 Published:2016-02-23

摘要:

考马斯亮蓝可与蛋白质生成蓝色复合物,经数码成像后,显色溶液的灰度差值和蛋白质质量浓度呈正比例,据此建立了数码成像比色法测定牛乳中蛋白质含量的方法,并对成像条件、显色条件、干扰物质进行了探讨。结果显示,用于牛乳中蛋白质含量测定时,该法的相对标准偏差小于2%,回收率在97.5%~102.6%之间,结果与考马斯亮蓝分光光度法一致,但更简便、快速。

关键词: 数码成像比色法, 蛋白质, 考马斯亮蓝

Abstract:

The grey value obtained by digital imaging for the blue complex formed from the binding interaction of
Coomassie brilliant blue with proteins is positively linearly related to the concentration of proteins. Thus, a method was
established for the determination of the protein content of milk by digital imaging colorimetry. The imaging conditions,
color development conditions and interfering substances were investigated. The recovery rates for spiked milk samples were
between 97.5% and 102.6% with a relative standard deviation (RSD) less than 2%. Consistent results were obtained using
digital imaging colorimetry and spectrophotometry, but digital imaging colorimetry was more convenient and faster.

Key words: digital imaging colorimetry, protein, Coomassie brilliant blue

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