基于LC-MS/MS的酸乳肽谱及ACE抑制活性

doi:10.7506/spkx1002-6630-201705034

食品科学

基于LC-MS/MS的酸乳肽谱及ACE抑制活性

于洋1,2，于文皓1,2，晏嘉泽2，董浩2，董家耕3，靳艳2,*，祁艳霞1,*

1.大连海洋大学食品科学与工程学院，辽宁大连 116023；2.中国科学院大连化学物理研究所，中国科学院分离分析化学重点实验室，辽宁大连 116023；3.辽宁辉山生物科技研究有限公司，辽宁沈阳 110031

出版日期:2017-03-15 发布日期:2017-03-28

Peptide Profiling of Yogurts Identified by LC-MS/MS and Their ACE Inhibitory Activities

YU Yang1,2, YU Wenhao1,2, YAN Jiaze2, DONG Hao2, DONG Jiageng3, JIN Yan2,*, QI Yanxia1,*

1. College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China; 2. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China; 3. Liaoning Huishan Biotechnology Research Co. Ltd., Shenyang 110031, China

Online:2017-03-15 Published:2017-03-28

摘要/Abstract

摘要： 利用液相色谱-串联质谱（liquid chromatography-tandem mass spectrometry，LC-MS/MS）的方法，对4 种酸乳（优味舒风味发酵乳、风味发酵乳、初乳24＋K风味发酵乳和十天风味发酵乳）的肽谱进行分析，分别鉴定到152、125、364 条和274 条肽段。利用血管紧张素转换酶（angiotensin converting enzyme，ACE）的抑制活性比较以上4 种酸乳的生物活性，在相同质量浓度2.0 mg/L时，4 种酸乳ACE抑制率分别为46.84%、42.75%、77.41%和63.84%。初乳24＋K风味发酵乳含有肽的数量大、ACE抑制活性高可能与其独特的原料成分相关。对4 种酸乳的肽谱及ACE抑制活性的考察，为进一步研究酸乳肽的功能特点和ACE抑制活性提供了理论依据。

关键词: 酸乳, 肽谱, 液相色谱-串联质谱, ACE抑制活性

Abstract: This study investigated the peptide profiling of four yogurt samples (Youweishu flavored fermented milk, plain flavored fermented milk, Churu 24+K flavored fermented milk and Shitian flavored fermented milk) from local market by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 152, 125, 364 and 274 peptides were identified from the yogurts by LC-MS/MS, respectively. Angiotensin converting enzyme (ACE) inhibitory activity was used to evaluate the biological activities of yogurt peptides. The ACE inhibition rates of peptides from these yogurts were 46.84%, 42.75%, 77.41% and 63.84% at a concentration of 2.0 mg/L, respectively. The large number of identified peptides and high ACE inhibitory activities in Churu 24+K flavor fermented milk may be related to its special ingredients. This study may provide the theoretical basis for further study on the functions and ACE inhibitory activity of yogurt peptides.

Key words: yogurt, peptide profiling, LC-MS/MS, ACE inhibitory activity

中图分类号:

TS252.54

于洋，于文皓，晏嘉泽，董浩，董家耕，靳艳，祁艳霞. 基于LC-MS/MS的酸乳肽谱及ACE抑制活性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201705034.

YU Yang, YU Wenhao, YAN Jiaze, DONG Hao, DONG Jiageng, JIN Yan, QI Yanxia. Peptide Profiling of Yogurts Identified by LC-MS/MS and Their ACE Inhibitory Activities[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201705034.

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基于LC-MS/MS的酸乳肽谱及ACE抑制活性

Peptide Profiling of Yogurts Identified by LC-MS/MS and Their ACE Inhibitory Activities

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