食品科学 ›› 2017, Vol. 38 ›› Issue (13): 90-95.doi: 10.7506/spkx1002-6630-201713015

• 基础研究 • 上一篇    下一篇

高压脉冲电场结合糖基化对β-乳球蛋白过敏原性与功能性质的影响

田 明,涂宗财,王 辉,杨文华,李 雪,宋启东   

  1. 1.南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047;2.江西师范大学生命科学学院,江西 南昌 330022
  • 出版日期:2017-07-15 发布日期:2017-07-11

Effect of Glycation Combined with Pulsed Electric Field Pretreatment on Allergenicity and Functional Properties of β-Lactoglobulin

TIAN Ming, TU Zongcai, WANG Hui, YANG Wenhua, LI Xue, SONG Qidong   

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; 2. College of Life Science, Jiangxi Normal University, Nanchang 330022, China
  • Online:2017-07-15 Published:2017-07-11

摘要: 采用高压脉冲电场(pulsed electric field,PEF)结合糖基化处理β-乳球蛋白(β-lactoglobulin,β-Lg),在β-Lg与半乳糖质量比1∶2、反应温度60 ℃、反应时间3 h、pH 7.4和不同PEF强度反应条件下,研究改性前后β-Lg的过敏原性和功能性质的变化。结果表明,经过PEF结合糖基化处理后,β-Lg自由氨基含量显著降低,β-Lg过敏原性有一定程度下降,15 kV/cm PEF处理90 μs后进行糖基化,过敏原性降低程度最大;在此条件下,β-Lg的溶解性、乳化性、乳化稳定性及抗氧化活性显著提高(P<0.05)。这为制备低致敏性且功能性质好的β-Lg提供了一种新的方法。

关键词: 脉冲电场, 糖基化, β -乳球蛋白, 过敏原性, 功能性质

Abstract: This research aimed to investigate the effect of pulsed electric field (PEF) pretreatment combined with glycation on the allergenicity and functional properties of β-lactoglobulin (β-Lg). After being subjected to PEF treatment at different electric intensities, β-Lg solution was added with galactose at a ratio of 1:2 and then they were allowed to react at pH 7.4 and 60 ℃ for 3 h. The results showed that glycation after PEF pretreatment significantly reduced the free amino group content of β-Lg and decreased the allergenicity. The lowest allergenicity of β-LG was observed by PEF pretreatment at 15 kV/cm for 90 μs, accompanied by a significant improvement in the solubility, emulsifying property, emulsion stability and antioxidant activity (P < 0.05). Therefore, glycation combined with PEF pretreatment will have potential application for producing β-Lg with low allergenicity and favorable functional properties.

Key words: pulsed electric field, glycation, β-lactoglobulin, allergenicity, functional properties

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