食品科学 ›› 1998, Vol. 19 ›› Issue (12): 42-45.

• 食品科学 • 上一篇    下一篇

不同提纯法精制的银杏叶提取物中黄酮糖苷的含量分析

 胡敏, 李晓丽, 张声华, 姜发堂   

  1. 华中农业大学食品科技系; 湖北工学院生物工程系
  • 出版日期:1998-12-15 发布日期:2011-12-13

 HU  Min, LI  Xiao-Li, ZHANG  Sheng-Hua, JIANG  Fa-Tang   

  • Online:1998-12-15 Published:2011-12-13

摘要: 用反相高效液相色谱法(RP-HPLC)分析了乙醇—水提取、水提取、柱层析及溶剂革取等方法精制的银杏叶总黄酮糖苷和水解后黄酮苷元的含量;用等梯度洗脱的方式成功地分辨出17个黄酮糖音;并定量分析了7个主要糖苷(含4个独特结构和活性的桂皮酰黄酮糖苷)的相对含量。结果表明:本法制备的GBE中,二种主要的桂皮酰黄酮糖苷的含量在总黄酮糖苷中所占的比例很高,可达50.34%,一般为35%左右。

关键词: HPLC, 提纯方法, GBE, 桂皮酰黄酮糖苷

Abstract: Using BP -HPLC, the total foaconoid glycoside contents, the proportions of six main flavone glycosides and the relative contents of the every typical flavonol coumaroyl ester glycosides in GBE prepared by four different extracting and purifing methods were analyzed. The result showed that the highest percentage of the two main flavonol coumaroyl ester glycosides in total flavonoid glycosides in GBE produced by our laboratory was 50. 34%, the average one was 35%.

Key words: HPLC, Extraction, Purification, GBE, Flavonol coumaroyl ester glycosides