食品科学 ›› 2000, Vol. 21 ›› Issue (12): 116-118.

• 分析检测 • 上一篇    下一篇

应用单磺酰氯柱前衍生法测定食品中的牛磺酸

 朱慧, 赵志红, 施文蓉   

  1. 杭州娃哈哈集团有限公司
  • 出版日期:2000-12-15 发布日期:2011-12-05

Determination of Taurine in Food by Pre-column HPLC with Sulfonyl Chloride

 ZHU  Hui, ZHAO  Zhi-Hong, SHI  Wen-Rong   

  • Online:2000-12-15 Published:2011-12-05

摘要: 提出柱前衍生高效液相色谱法测定饮料中的牛磺酸。在碱性条件下,用单磺酰氯作为衍生化试剂,以乙腈—醋酸钠缓冲溶液(pH=7.2)为流动相,在290nm处进行紫外检测。

关键词: 柱前衍生, 牛磺酸, 高效液相色谱, 单磺酰氯

Abstract: This paper presented a method for determining taurine by pre-column high performance liquid chromatography (HPLC) in alkaline condition. Sulfonyl chloride was used as derivatization agent. The derivation is detected by C18 reversed phase HPLC column UV 290nm with acetonitrile sodium acetate (pH=7.2) as buffer in mobile phase.

Key words: Taurine Pre-column derivatization HPLC Sulfonyl chlor8