关键词: 高效液相色谱, 蔬菜, 异构体, 白藜芦醇苷, 白藜芦醇, 异丙醇, 荧光检测器

Abstract: Objecive:A sensitive and rapid high-performance liquid chromatography with fluorescence detector method was developed for the determination of the (E)-and (Z)-diastereomers of resveratrol and resveratrol glucoside in vegetables. Methods: Chromatographic separation was achieved with a NUCLEODUR 100-5 C18 column(250mm×4.6mm, 5μm) while a mobile phase program of gradient elution with is-propyl alcohol and water at a flow rate of 0.6ml/min was employed with fluorescence detector: ex: 260nm and em: 472nm. Results: Under the optimum conditions for every component, the limit of detection of the (Z)-and (E)-diastereomers of resveratrol glucoside and resveratrol was 0.102, 0.117, 0.083 and 0.084 ng respectively at a signal-to-noise 3:1. The linear range was 0.004～1.0mg/L.The average recoveries of the standards added were 94.7% and 101.1%, for the (E)- diastereomers of resveratrol and resveratrol glucoside respectively, and 90.7%, 92.4% for the (Z)-diastereomers of resveratrol and resveratrol glucoside respectively, whereas relative standard deviations were within the range of 0.92%～1.32%, 1.25%～ 1.94% respectivtly. Conclusion: This method is accurate and reliable for the quantitive analysis of the (E)-and (Z)-diastereomers of resveratrol and resveratrol glucoside in vegetables.

Key words: RP-HPLC, vegetables, diastereomers, resveratrol, resveratrol glucoside, iso-propyl alcohol, fluorescence detector