关键词: 香兰素, 姜黄素, 提取, 吸附, 高效液相色谱法

Abstract: The technics of extracting the vanillin from curry powder was researched,and the technical flow which using the Indian curry powder as a raw material to extract the curcumin then invert it to the vanillin was determined in the paper.Using 95 percent of alcohol as extracting reagent to extract the curcumin,an optimized extraction condition was obtained through the single factor tests:temperature 65 ℃,time 1.5 h,ratio of solid to solvent 1:25,extracting 2 times,the extraction percentage reaches 95.7%.The crude curcumin product was adsorbed by using macroporous resin for purification.Through static adsorption and desorption experiments,the sorption and desorption properties of both D-101 and CAD-40 macroporous resins were studied.The results showed that the adsorption and desorption capacity of D-101 resin was better than CAD-40,so D-101 resin was chosen to purify curcumin.Then,curcumin was transformed into the vanillin by acid and base treatments,and was purified by sodium bisulfite.0.15g of the end product was obtained,and the total yield is 3.6%.Finally,the contents of vanillin in the end product are determined by HPLC method.The results showed that the concentration of vanillin obtained is 0.00986 mol/L,and the purity is 90.71%.

Key words: vanillin, curcumin, extracts, adsorption, HPLC