高效液相色谱法测定紫菜中牛磺酸含量

食品科学 ›› 2012, Vol. 33 ›› Issue (18): 162-164.

高效液相色谱法测定紫菜中牛磺酸含量

王芬，张婷，顾振新，陈志刚*

南京农业大学食品科技学院，农业部农畜产品加工与质量控制重点开放实验室

收稿日期:2011-08-03 修回日期:2012-07-25 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: 陈志刚 E-mail:zgchen@njau.edu.cn
基金资助:
中央高校基本科研业务费专项资金;教育部博士点（新教师）基金;江苏省自然科学基金

Determination of Taurine in Porphyra yzoensis by High Performance Liquid Chromatography

Received:2011-08-03 Revised:2012-07-25 Online:2012-09-25 Published:2012-09-07

摘要/Abstract

摘要： 采用高效液相色谱(HPLC)法测定紫菜中牛磺酸含量。用邻苯二甲醛进行柱前衍生，以XDB C18 (150mm×4.6mm，5μm)色谱柱为分析柱，甲醇- pH5.3醋酸盐缓冲液(30:70)为流动相，流速1.0mL/min，检测波长315nm，温度30℃。牛磺酸质量浓度在5.0～300.0μg/mL范围内线性关系良好(R2＝0.9991)，平均回收率(n＝6)为98.8%，相对标准偏差为1.8%，衍生物在15min内的稳定性良好，最低检出限0.25μg/mL。用本研究建立的HPLC法测定紫菜中牛磺酸含量为(1.04±0.07)%(干质量)。用此方法测定牛磺酸含量不受紫菜中其他成分干扰，简单、快速、专属性强，灵敏度高，结果准确、可靠。

关键词: 高效液相色谱, 柱前衍生, 牛磺酸, 紫菜

Abstract: An HPLC method was proposed to determine the content of taurine in Porphyra yzoensis. Taurine was subjected to pre-column derivatization with OPA. The separation was performed on a XDB C18 (150 mm × 4. 6 mm, 5 μm) using a mobile phase composed of methanol and pH 5.3 sodium acetate buffer (30:70) at a flow rate of 1 mL/min. Taurine was detected at a 315 nm wavelength. The column temperature was set at 30 ℃. Taurine was well separated with a good linear relationship in the range of 5.0－300.0μg/mL (R2 = 0.9991). The average recovery was 98.8% (n = 6) with a relative standard deviation of 1.8%. The taurine derivative was stable within 15 min. The limit of detection for taurine was 0.25μg/mL. The propose HPLC method was successfully applied to determine taurine in Porphyra yzoensis with a result of (1.04 ± 0.07)% (dry wt). This method was interference-free, simple, rapid, specific, sensitive, accurate and reliable.

Key words: high performance liquid chromatography, pre-column derivatization, taurine, Porphyra yzoensis

中图分类号:

TS201.7

王芬，张婷，顾振新，陈志刚*. 高效液相色谱法测定紫菜中牛磺酸含量[J]. 食品科学, 2012, 33(18): 162-164.

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