HPLC-ABTS+在线法筛选细叶杜香抗氧化活性成分

食品科学 ›› 2012, Vol. 33 ›› Issue (19): 88-91.

HPLC-ABTS+在线法筛选细叶杜香抗氧化活性成分

裴世春1，徐玖亮2，UM Byunghun2，商亚芳2

1. 齐齐哈尔大学食品与生物工程学院 2. 韩国KIST江陵分院天然产物研究中心

收稿日期:2012-06-26 修回日期:2012-08-28 出版日期:2012-10-15 发布日期:2012-09-17
通讯作者: 裴世春 E-mail:peisc2002cn@yahoo.com.cn
基金资助:
黑龙江省教育厅科学技术研究（面上）项目

Screening of Antioxidant Components from Ledum palustre L. Leaves by Online HPLC-ABTS+ Method

Received:2012-06-26 Revised:2012-08-28 Online:2012-10-15 Published:2012-09-17
Contact: Shi-Chun PEI E-mail:peisc2002cn@yahoo.com.cn

摘要/Abstract

摘要： 利用HPLC-ABTS＋·在线法对细叶杜香叶中的抗氧化活性成分进行筛选和分析鉴定，结果表明：在细叶杜香甲醇提取物的乙酸乙酯萃取组分中筛选到2种抗氧化活性成分，经质谱和核磁共振波谱分析鉴定为染料木苷和槲皮素。

关键词: 细叶杜香叶, HPLC-ABTS＋·, 在线检测, 抗氧化活性, 核磁共振, 质谱

Abstract: In this study, antioxidant compounds from Ledum palustre L. leaves were screened and identified by using an online high-performance liquid chromatography (HPLC)-ABTS+· screening system. Two antioxidant compounds from the ethyl acetate fraction in methanol extract were obtained. By mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectroscopy, both compounds were identified as genistin and quercetin, respectively.

Key words: Ledum palustre L., HPLC-ABTS+·, screening system, online detection, antioxidant activity, NMR, MS

中图分类号:

R446.61

裴世春1，徐玖亮2，UM Byunghun2，商亚芳2. HPLC-ABTS+在线法筛选细叶杜香抗氧化活性成分[J]. 食品科学, 2012, 33(19): 88-91.

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