食品科学 ›› 2019, Vol. 40 ›› Issue (19): 185-195.doi: 10.7506/spkx1002-6630-20180818-189

• 营养卫生 • 上一篇    下一篇

基于代谢组学研究荡秋千运动下普洱茶茶褐素对大鼠代谢综合征改善作用

吴恩凯,张婷婷,彭春秀,王秋萍,谭超,龚加顺   

  1. (1.云南农业大学食品科学技术学院,云南 昆明 650201;2.云南农业大学园林园艺学院,云南 昆明 650201)
  • 出版日期:2019-10-15 发布日期:2019-10-25
  • 基金资助:
    国家自然科学基金地区科学基金项目(31560456);云南省农业基础研究联合专项(2017FG001-011)

Influence of Swinging on the Metabolome of Pu-erh Tea Theabrownins in Rats Fed with High-Fat, High-Sugar, High-Salt Diet

WU Enkai, ZHANG Tingting, PENG Chunxiu, WANG Qiuping, TAN Chao, GONG Jiashun   

  1. (1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2. College of Horticulture and Landscape, Yunnan Agricultural University, Kunming 650201, China)
  • Online:2019-10-15 Published:2019-10-25

摘要: 分析比较荡秋千运动对普洱茶茶褐素(theabrownin,TB)在高脂高糖高盐饮食大鼠血清内的代谢组学变化。采用1H-核磁共振(1H-nuclear magnetic resonance,1H-NMR)代谢组学技术对14 组大鼠的血清进行分析,归属、指认图谱中化合物,进行正交偏最小二乘-判别分析(orthogonal partial least squares-discrimination analysis,OPLS-DA),发现其中的差异及规律。结果显示:常规代谢综合征(metabolic syndrome,MS)模型组大鼠血清中异亮氨酸、亮氨酸、缬氨酸、3-羟基丁酸相对含量显著低于常规正常对照组(P<0.05),丙氨酸、N-乙酰基-糖蛋白、谷氨酸、二甲基甘氨酸、甜菜碱、牛磺酸、精氨酸、糖原、甘油相对含量则显著高于常规正常对照组(P<0.05)。荡秋千TB高剂量组大鼠的3-羟基丁酸相对含量显著高于荡秋千MS模型组,而低密度脂蛋白/极低密度脂蛋白(low density lipoprotein/very low density lipoprotein,LDL/VLDL)、乳酸、丙氨酸、精氨酸相对含量则显著低于荡秋千MS模型组。与常规正常对照组相比,荡秋千后的正常对照组大鼠血清中高密度脂蛋白(high density lipoprotein,HDL)、异亮氨酸、3-羟基丁酸相对含量显著提高,而二甲基甘氨酸、精氨酸、糖原、α-葡萄糖相对含量则显著降低(P<0.05)。与MS模型组相比,荡秋千MS模型组大鼠血清中LDL/VLDL、异亮氨酸相对含量显著降低,而丙氨酸、乳酸相对含量则显著升高(P<0.05)。研究结果表明,荡秋千可明显降低血清LDL/VLDL相对含量,升高HDL相对含量,促进血糖代谢;荡秋千运动与TB处理具有协同增效的作用,在预防代谢综合征和心脑血管疾病方面具有很大潜力。

关键词: 荡秋千运动, 普洱茶茶褐素, 代谢组学, 代谢综合征, 大鼠

Abstract: The swinging-induced metabolomic changes of theabrownins (TB) extracted from Pu-erh tea in the serum of rats fed with a high-fat, high-sugar, high-salt diet were investigated by 1H-nuclear magnetic resonance (1H-NMR). An NMR-based metabolomic approach was used to analyze the NMR spectra of sera from 14 rat groups. Orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to analyze the obtained metabolites in the comparison of chemical constitutes from different groups. The results showed that before swinging, the relative contents of isoleucine, leucine, valine and 3- hydroxybutyrate decreased significantly in serum from the metabolic syndrome (MS) model group (P < 0.05) compared with the normal control group, while the relative contents of alanine, N-acetyl-glycoprotein, glutamic acid, dimethylglycine (DMG), betaine, taurine, arginine, glycogen, glycerol increased significantly (P < 0.05). After swinging, compared with the MS model group, the relative content of 3-hydroxybutyrate in the high-dose TB group increased significantly (P < 0.05), while low density lipoprotein/very low density lipoprotein (LDL/VLDL) ratio and the relative contents of lactic acid, alanine and arginine decreased significantly (P < 0.05). After swinging, the relative contents of serum HDL, isoleucine and 3-hydroxybutyrate in the normal control group significantly increased (P < 0.05), while the relative contents of dimethylglycine (DMG), arginine, glycogen, and α-glucose were significantly decreased (P < 0.05). For the MS model group, LDL/VLDL ratio and the relative content of isoleucine in serum decreased significantly (P < 0.05) after swinging, while the relative contents of alanine and lactic acid increased significantly (P < 0.05). The metabolomic results demonstrated that swinging could significantly reduce the level of serum LDL/VLDL ratio and increase HDL level, and effectively promote blood glucose metabolism. Swinging and TB have a synergistic effect in rats with MS, and have great potential in the prevention of metabolic syndromes and cardiovascular diseases.

Key words: swinging, Pu-erh tea theabrownins, metabolomics, metabolic syndromes, rats

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