高效液相色谱法测定酱香型白酒中挥发性酚类物质

doi:10.7506/spkx1002-6630-201224050

食品科学 ›› 2012, Vol. 33 ›› Issue (24): 239-243.doi: 10.7506/spkx1002-6630-201224050

高效液相色谱法测定酱香型白酒中挥发性酚类物质

寻思颖1，董睿2，彭黔荣3，冯永渝1，杨敏2,*，孙棣1，耿平兰1

1.国家酒类及饮料产品质量监督检验中心，贵州贵阳 550004；2.贵州大学化学与化工学院，贵州贵阳 550003；3.贵州中烟工业有限责任公司贵阳卷烟厂，贵州贵阳 550003

收稿日期:2011-10-27 修回日期:2012-11-15 出版日期:2012-12-25 发布日期:2012-12-12
通讯作者: 杨敏 E-mail:ce.myang@gzu.edu.cn

Determination of Volatile Phenols in Maotai-Flavor Liquors by HPLC-FLD

XUN Si-ying1，DONG Rui2，PENG Qian-rong3，FENG Yong-yu1，YANG Min2,*，SUN Di1，GENG Ping-lan1

1. National Centre of Quality Supervision and Detection for Wine and Beverages, Guiyang 550004, China；2. School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550003, China；3. Guiyang Cigarettes Factory, China Tobacco Guizhou Industrial Co. Ltd., Guiyang 550003, China

Received:2011-10-27 Revised:2012-11-15 Online:2012-12-25 Published:2012-12-12

摘要/Abstract

摘要： 利用酚类物质的荧光特性和手性拆分剂β-环糊精拆分重叠峰，建立带荧光检测-高效液相色谱仪法测定酱香型白酒中10种挥发性酚类物质含量的方法，色谱条件如下：色谱柱，Synergi 4u Hydro-RP 80A (150mm×4.6mm，4μm)；荧光检测器波长：Ex=270nm，Em=315nm；流动相A：冰醋酸-β-环糊精的水溶液(1:100，V/V)；流动相B：冰醋酸-乙腈-β-环糊精的水溶液(1:40:60，V/V)，梯度洗脱分析。该法对10种挥发性酚类化合物测定的回收率为83.8%～101.6%，相对标准偏差为0.9%～2.5%，检出限为0.0025～0.0506μg/mL。说明该方法准确度、灵敏度和重复性好。

关键词: 高效液相色谱, 挥发性酚, β-环糊精, 酱香型白酒

Abstract: A high-performance liquid chromatography with fluorescence detector (HPLC-FLD) method for the determination of volatile phenols in Maotai-flavor liquors was established. This method was based on the fluorescent nature of volatile phenols and the separation of overlapped peaks with β-cyclodextrin as a resolving agent. The chromatographic separation was achieved on Synergi 4u Hydro- RP 80A column (150 mm × 4.6 mm, 4 μm) using acetic acid-β-cyclodextrin aqueous solution of (1:100, V/V) as mobile phase A and acetic acid-acetonitrile-β-cyclodextrin aqueous solution (1:40:60, V/V) by gradient elution. The excitation and emission wavelengths were set as 270 nm and 315 nm, respectively. Average recoveries of 10 volatile phenols in liquors were 83.8%–101.6% with RSDs ranging from 0.9% to 2.5%. The limits of detection of this method were 0.0025–0.0506 μg/mL. These results indicated that this method was accurate, sensitive and repeatable.

Key words: HPLC, volatile phenols, β-cyclodextrin, Maotai-flavor liquors

中图分类号:

TS207.3

寻思颖1，董睿2，彭黔荣3，冯永渝1，杨敏2,*，孙棣1，耿平兰1. 高效液相色谱法测定酱香型白酒中挥发性酚类物质[J]. 食品科学, 2012, 33(24): 239-243.

参考文献

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高效液相色谱法测定酱香型白酒中挥发性酚类物质

Determination of Volatile Phenols in Maotai-Flavor Liquors by HPLC-FLD

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