食品科学 ›› 2013, Vol. 34 ›› Issue (7): 148-151.doi: 10.7506/spkx1002-6630-201307031

• 生物工程 • 上一篇    下一篇

品质改良因子对液体发酵培养平菇菌丝体的生物量和抗氧化系统的影响

王翠萍1,生吉萍2,*,申 琳1,*   

  1. 1.中国农业大学食品科学与营养工程学院,北京 100083;2.中国人民大学农业与农村发展学院,北京 100872
  • 收稿日期:2012-01-05 修回日期:2013-02-27 出版日期:2013-04-15 发布日期:2013-03-20
  • 通讯作者: 申琳 E-mail:shen5000@cau.edu.cn
  • 基金资助:
    国家自然科学基金面上项目

Effects of Quality Modification Factor from Stalk on Biomass and Antioxidant Systems of Liquid-Cultivated Mycelia of Pleurotus ostreatus

WANG Cui-ping1,SHENG Ji-ping2,*,SHEN Lin1,*   

  1. 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. School of Agricultural Economics and Rural Development, Renmin University of China, Beijing 100872, China
  • Received:2012-01-05 Revised:2013-02-27 Online:2013-04-15 Published:2013-03-20
  • Contact: SHEN Lin E-mail:shen5000@cau.edu.cn

摘要: 以平菇(Pleurotus ostreatus) F5为实验材料,研究其在不同体积分数品质改良因子培养7d后,菌丝生物量的变化,以及添加体积分数0.5%品质改良因子后,不同发酵时期菌丝体中DPPH自由基和羟自由基(·OH)清除率、总抗氧化能力(T-AOC)及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)4个抗氧化相关酶活性的变化。结果表明:与对照组相比,0.5%品质改良因子处理组菌丝体干质量提高了19.31%,DPPH自由基清除率无明显变化,·OH清除率在5~7d提高了8%~11%,培养前5d菌丝体的总抗氧化能力均高于对照,在第5天的提高了32%,并且0.5%的品质改良因子处理组的菌丝体 SOD、POD、CAT、APX活性在培养的3~5d均高于对照组(P<0.05),说明添加0.5%品质改良因子培养的平菇不仅能够提高生物产量还能够显著提高菌丝体的抗氧化能力。

关键词: 平菇, 品质改良因子, 液体培养, 抗氧化

Abstract: This study aimed to investigate the effect of different concentrations of stalk-derived quality modification factor (QMF) solution on the biomass of Pleurotus ostreatus mycelia over a 7-day culture period and the effects of 0.5% QMF solution on dynamic changes in DPPH and hydroxyl radical scavenging capacity, total antioxidant activity (T-AOC) and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX). The results showed that the 0.5% QMF treatment led to an increase in the mycelial dry weight of Pleurotus ostreatus by 19.31% on the 7th day compared with the control group. Additionally, despite no marked change in the DPPH radical scavenging activity, there was an 8%—11% increase in the hydroxyl radical scavenging activity after cultivation for 5, 6, 7 days, and an increase in the total antioxidant capacity up to 32% on the fifth day was also found during the first 5 days of cultivation compared with the control group. Moreover, the activities of SOD, POD, CAT and APX after cultivation for 3, 4, 5 days showed increased levels compared with the control group (P < 0.05). These observations show that treatment with 0.5% QMF solution can increase the mycelial biomass while apparently enhancing the antioxidant activity of Pleurotus ostreatus mycelia.

Key words: Pleurotus ostreatus, quality modification factor from stalk, liquid-state cultivation, antioxidation

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