响应面分析优化菊粉的羧甲基改性工艺

食品科学 ›› 2012, Vol. 33 ›› Issue (18): 63-66.

响应面分析优化菊粉的羧甲基改性工艺

李丹丹1，王雪娇1，周杰2，潘烨1，王爽1，高学佳1

1.河北科技大学生物科学与工程学院 2.贵州大学生命科学学院

收稿日期:2011-07-06 修回日期:2012-07-08 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: 李丹丹 E-mail:dandan_jiejie@yahoo.com.cn

Optimization of Inulin Carboxymethylation by Response Surface Analysis

Received:2011-07-06 Revised:2012-07-08 Online:2012-09-25 Published:2012-09-07
Contact: Dan-Dan LI E-mail:dandan_jiejie@yahoo.com.cn

摘要/Abstract

摘要： 研究菊粉的羧甲基改性工艺条件，以碱化时间、醚化温度、醚化时间、碱用量以及氯乙酸用量为影响因素，以羧甲基的取代度为考察指标，运用Plackett-Burman设计筛选出3个对菊粉羧甲基取代度影响显著因素，即碱化时间、醚化温度和氯乙酸用量，采用响应面分析试验优化菊粉的羧甲基改性工艺。菊粉的羧甲基改性最优工艺条件为菊粉用量6.8g、碱化时间30min、醚化温度81℃、氯乙酸用量2.96g，此时菊粉的羧甲基取代度为0.66±0.000167。

关键词: 菊粉, 羧甲基改性, Plackett-Burman试验, 响应面分析

Abstract: In order to find optimal conditions for the the carboxymethylation of inulin, we investigated the effects of amount of NaOH used for alkalization reaction, alkalization time, amount of chloroacetic acid used for etherification, and etherification temperature and time on substitution degree of carboxymethyl inulin. Using Plackett-Burman design, we identified alkalization time, etherification temperature and chloroacetic acid amount as important conditions that influence substitution degree of carboxymethyl inulin. Response surface methodology was employed to optimize the important conditions. Optimal carboxymethylation of inulin was achieved by alkalization for 30 min and etherification with 2.96 g of chloroacetic acid per 6.8 g of inulin at 81 ℃. The substitution degree of the obtained carboxymethyl inulin was 0.66 ± 0.000167.

Key words: inulin, carboxymethylation, Plackett-Burman design, response surface methodology

中图分类号:

TS213.2

李丹丹1，王雪娇1，周杰2，潘烨1，王爽1，高学佳1. 响应面分析优化菊粉的羧甲基改性工艺[J]. 食品科学, 2012, 33(18): 63-66.

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